HYKK - Hydroxylysine kinase - human protein (Function)
 
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Protein
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References

 
HYKK »  Hydroxylysine kinase   [ EC 2.7.1.81 ]  (5-hydroxy-L-lysine kinase)
 
Protein also known as:  Aminoglycoside phosphotransferase domain-containing protein 1.
Gene name:  HYKK
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine.  
1
  • CuratedUniProtKB
GO molecular function 
Hydroxylysine kinase activitydefinition[GO:0047992]  
  • IEAEC 2 GO
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.7.1.81: GTP + 5-hydroxy-L-lysine GDP + 5-phosphonooxy-L-lysine.  
1
  • CuratedUniProtKB
 

Biophysicochemical properties

Kinetic parameters
KM 280 uM for (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP)
KM 3.5 uM for GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)
KM 7.6 uM for (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP)
KM 810 uM for ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)
Vmax 0.49 umol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 0.2 mM GTP)
Vmax 4.95 umol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (in the presence of 2 mM ATP)
Vmax 0.57 umol/min/mg enzyme toward GTP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)
Vmax 5.28 umol/min/mg enzyme toward ATP (in the presence of 0.5 mM (5R)-5-hydroxy-L-lysine)

Keywords

Molecular function 
Kinase  definition   [KW-0418]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GenomeRNAi: 123688
PRO: PR:A2RU49