POLR3A - DNA-directed RNA polymerase III subunit RPC1 - human protein (Function)
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POLR3A »  DNA-directed RNA polymerase III subunit RPC1   [ EC ]  (RNA polymerase III subunit C1)
Protein also known as:  DNA-directed RNA polymerase III largest subunit.
Gene name:  POLR3A
Entry whose protein(s) existence is based on evidence at protein level
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DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.  
  • UniProtKB
GO molecular function 
Chromatin bindingdefinition[GO:0003682] silver  
  • Ortholog Compara
DNA bindingdefinition[GO:0003677] silver  
  • InterPro 2 GO
DNA-directed RNA polymerase activitydefinition[GO:0003899]  
  • UniProtKB
Metal ion bindingdefinition[GO:0046872]  
  • UniProtKB KW
Contributes to RNA polymerase III activitydefinition[GO:0001056]  
  • RefGenome
GO biological process 
Defense response to virusdefinition[GO:0051607]  
  • UniProtKB KW
Innate immune responsedefinition[GO:0045087]  
  • UniProtKB
Positive regulation of interferon-beta productiondefinition[GO:0032728]  
  • UniProtKB
Positive regulation of protein targeting to mitochondriondefinition[GO:1903955]  
  • ParkinsonsUK-UCL
Regulation of mitophagydefinition[GO:1903146]  
  • ParkinsonsUK-UCL
Transcription, DNA-templateddefinition[GO:0006351]  
  • UniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC Nucleoside triphosphate + RNA(n) diphosphate + RNA(n+1).  
  • UniProtKB
According to KEGG, this protein belongs to the following pathways:
Cytosolic DNA-sensing pathway  hsa04623+11128  
Metabolic pathways  hsa01100+11128  
Purine metabolism  hsa00230+11128  
Pyrimidine metabolism  hsa00240+11128  
RNA polymerase  hsa03020+11128  
According to Reactome, this protein belongs to the following pathways:
Cytosolic sensors of pathogen-associated DNA  R-HSA-1834949  
RNA Polymerase III Abortive And Retractive Initiation  R-HSA-749476  
RNA Polymerase III Chain Elongation  R-HSA-73780  
RNA Polymerase III Transcription Initiation From Type 1 Promoter  R-HSA-76061  
RNA Polymerase III Transcription Initiation From Type 2 Promoter  R-HSA-76066  
RNA Polymerase III Transcription Initiation From Type 3 Promoter  R-HSA-76071  
RNA Polymerase III Transcription Termination  R-HSA-73980  


Biological process 
Antiviral defense  definition   [KW-0051]
Immunity  definition   [KW-0391]
Innate immunity  definition   [KW-0399]
Transcription  definition   [KW-0804]
Molecular function 
Nucleotidyltransferase  definition   [KW-0548]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]

Further external links

GenomeRNAi: 11128
PRO: PR:O14802