HPGDS - Hematopoietic prostaglandin D synthase - human protein (Function)
 
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HPGDS »  Hematopoietic prostaglandin D synthase   [ EC 5.3.99.2 ]  (H-PGDS)
 
Protein also known as:  Glutathione-requiring prostaglandin D synthase.
Gene name:  HPGDS
Family name: GST » Sigma
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Bifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides and organic isothiocyanates. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide.  
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  • UniProtKB
GO molecular function 
Calcium ion bindingdefinition[GO:0005509]  
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  • UniProtKB
Glutathione transferase activitydefinition[GO:0004364]  
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  • EC 2 GO
Magnesium ion bindingdefinition[GO:0000287]  
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  • UniProtKB
Prostaglandin-D synthase activitydefinition[GO:0004667]  
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  • UniProtKB
Protein homodimerization activitydefinition[GO:0042803]  
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  • UniProtKB
GO biological process 
Locomotory behaviordefinition[GO:0007626]  
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  • PINC
Prostaglandin metabolic processdefinition[GO:0006693]  
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  • UniProtKB
Signal transductiondefinition[GO:0007165]  
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  • PINC
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC 5.3.99.2: (5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.  
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  • UniProtKB
EC 2.5.1.18: RX + glutathione HX + R-S-glutathione.  
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  • UniProtKB
It requires the following cofactor
 glutathione : Glutathione is required for the prostaglandin D synthase activity.  
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  • UniProtKB
It is regulated in the following manner
Prostaglandin PGD2 synthesis is stimulated by calcium and magnesium ions. One calcium or magnesium ion is bound between the subunits of the homodimer. The interactions with the protein are for the most part mediated via water molecules. Magnesium increases the affinity for glutathione, while calcium has no effect on the affinity for glutathione.  
2
  • UniProtKB
Pathways 
According to KEGG, this protein belongs to the following pathways:
Arachidonic acid metabolism  hsa00590+27306  
Metabolic pathways  hsa01100+27306  
According to Reactome, this protein belongs to the following pathways:
Glutathione conjugation  REACT_6926  
Synthesis of Prostaglandins (PG) and Thromboxanes (TX)  REACT_150149  
 
More information is available from:
 

Biophysicochemical properties

Kinetic parameters
KM 8 mM for glutathione for the glutathione-conjugating activity
KM 0.6 mM for glutathione for the prostaglandin D synthase activity in the presence of EDTA
KM 0.14 mM for glutathione for the prostaglandin D synthase activity in the presence of magnesium ions
Vmax 0.05 umol/min/mg enzyme with cumene hydroperoxide as substrate
Vmax 5.1 umol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate
Vmax 6.8 umol/min/mg enzyme with allyl isothiocyanate as substrate
Vmax 8.6 umol/min/mg enzyme with 1-bromo-2,4-dinitrobenzene as substrate
Vmax 44.3 umol/min/mg enzyme with 1-fluoro-2,4-dinitrobenzene as substrate
Vmax 6.3 umol/min/mg enzyme with benzyl isothiocyanate as substrate
Vmax 10.7 umol/min/mg enzyme with 1-iodo-2,4-dinitrobenzene as substrate

Keywords

Biological process 
Fatty acid biosynthesis  definition   [KW-0275]
Fatty acid metabolism  definition   [KW-0276]
Lipid biosynthesis  definition   [KW-0444]
Lipid metabolism  definition   [KW-0443]
Prostaglandin biosynthesis  definition   [KW-0643]
Prostaglandin metabolism  definition   [KW-0644]
Molecular function 
Isomerase  definition   [KW-0413]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: O60760
Other
GeneWiki: PGDS
GenomeRNAi: 27306
PRO: PR:O60760
Chemistry
GuidetoPHARMACOLOGY: 1381