FARS2 - Phenylalanine--tRNA ligase, mitochondrial - human protein (Function)
 
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FARS2 »  Phenylalanine--tRNA ligase, mitochondrial
 
Gene name:  FARS2
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.  
2
  • CuratedUniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
Magnesium ion bindingdefinition[GO:0000287] silver  
  • IEAInterPro 2 GO
Phenylalanine-tRNA ligase activitydefinition[GO:0004826]  
1
  • IDAUniProtKB
tRNA bindingdefinition[GO:0000049]  
1
  • IDAUniProtKB
GO biological process 
Phenylalanyl-tRNA aminoacylationdefinition[GO:0006432]  
1
  • IDAUniProtKB
tRNA processingdefinition[GO:0008033]  
1
  • IDAUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 6.1.1.20: ATP + L-phenylalanine + tRNA(Phe) AMP + diphosphate + L-phenylalanyl-tRNA(Phe).  
2
  • CuratedUniProtKB
Pathways 
According to KEGG, this protein belongs to the following pathway:
Aminoacyl-tRNA biosynthesis  hsa00970+10667  
According to Reactome, this protein belongs to the following pathway:
Mitochondrial tRNA aminoacylation  REACT_15302  
 

Biophysicochemical properties

Kinetic parameters
kcat is 2.8 min(-1), 2.0 min(-1) and 3.1 min(-1) with L-phenylalanine, L-tyrosine and m-tyrosine as substrate, respectively. Thus, the catalytic efficiency of the m-Tyr attachment is only 5-fold lower than that of the correct amino acid, while that of Tyr attachment is 1000-fold lower (PubMed:19549855).
KM 2.9 mM for ATP (PubMed:22833457)
KM 7.3 uM for L-phenylalanine (PubMed:22833457)
KM 11.7 uM for DL-m-tyrosine (PubMed:19549855)
KM 1900 uM for L-tyrosine (PubMed:19549855)
KM 2.2 uM for L-phenylalanine (PubMed:19549855)
KM 1.2 uM for tRNA(Phe) (PubMed:22833457)

Keywords

Biological process 
Protein biosynthesis  definition   [KW-0648]
Molecular function 
Aminoacyl-tRNA synthetase  definition   [KW-0030]
Ligase  definition   [KW-0436]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: FARS2
GenomeRNAi: 10667
PRO: PR:O95363