HPRT1 - Hypoxanthine-guanine phosphoribosyltransferase - human protein (Function)
 
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HPRT1 »  Hypoxanthine-guanine phosphoribosyltransferase   [ EC 2.4.2.8 ]  (HGPRT)
 
Gene name:  HPRT1
Entry whose protein(s) existence is based on evidence at protein level
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Function

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Overview 
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.  
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  • UniProtKB
GO molecular function 
Guanine phosphoribosyltransferase activitydefinition[GO:0052657]  
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  • UniProtKB
Hypoxanthine phosphoribosyltransferase activitydefinition[GO:0004422]  
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  • UniProtKB
Magnesium ion bindingdefinition[GO:0000287]  
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  • UniProtKB
Nucleotide bindingdefinition[GO:0000166]  
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  • UniProtKB KW
Protein bindingdefinition[GO:0005515]  
2
  • IntAct
Protein homodimerization activitydefinition[GO:0042803]  
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  • UniProtKB
GO biological process 
Adenine salvagedefinition[GO:0006168]  
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  • RefGenome
Central nervous system neuron developmentdefinition[GO:0021954] silver  
1
  • Ortholog Compara
Cerebral cortex neuron differentiationdefinition[GO:0021895] silver  
1
  • Ortholog Compara
Cytolysisdefinition[GO:0019835] silver  
1
  • Ortholog Compara
Dendrite morphogenesisdefinition[GO:0048813] silver  
1
  • Ortholog Compara
Dopamine metabolic processdefinition[GO:0042417] silver  
1
  • Ortholog Compara
GMP catabolic processdefinition[GO:0046038]  
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  • UniProtKB
GMP salvagedefinition[GO:0032263]  
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  • RefGenome
Grooming behaviordefinition[GO:0007625] silver  
1
  • Ortholog Compara
Guanine salvagedefinition[GO:0006178]  
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  • UniProtKB
Hypoxanthine metabolic processdefinition[GO:0046100]  
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  • UniProtKB
Hypoxanthine salvagedefinition[GO:0043103]  
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  • UniProtKB
IMP metabolic processdefinition[GO:0046040]  
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  • UniProtKB
IMP salvagedefinition[GO:0032264]  
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  • UniPathway
  • RefGenome
Locomotory behaviordefinition[GO:0007626] silver  
1
  • Ortholog Compara
Lymphocyte proliferationdefinition[GO:0046651] silver  
1
  • Ortholog Compara
Positive regulation of dopamine metabolic processdefinition[GO:0045964]  
1
  • UniProtKB
Protein homotetramerizationdefinition[GO:0051289]  
2
  • UniProtKB
Purine nucleotide biosynthetic processdefinition[GO:0006164]  
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  • UniProtKB
Purine ribonucleoside salvagedefinition[GO:0006166]  
1
  • UniProtKB
Response to amphetaminedefinition[GO:0001975] silver  
1
  • Ortholog Compara
Striatum developmentdefinition[GO:0021756] silver  
1
  • Ortholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
GMP + diphosphate guanine + 5-phospho-alpha-D-ribose 1-diphosphate.  
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  • UniProtKB
EC 2.4.2.8: IMP + diphosphate hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.  
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  • UniProtKB
It requires the following cofactor
 Mg(2+) : Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.  
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  • UniProtKB
Pathways 
This protein is involved in the following pathway
Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1  
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  • UniProtKB
According to KEGG, this protein belongs to the following pathways:
Drug metabolism - other enzymes  hsa00983+3251  
Metabolic pathways  hsa01100+3251  
Purine metabolism  hsa00230+3251  
According to Reactome, this protein belongs to the following pathway:
Purine salvage  REACT_1923  
 
More information is available from:
 

Biophysicochemical properties

Kinetic parameters
KM 0.45 uM for hypoxanthine
KM 25 uM for pyrophosphate
KM 5.4 uM for IMP
KM 31 uM for phosphoribosylpyrophosphate

Keywords

Biological process 
Purine salvage  definition   [KW-0660]
Molecular function 
Glycosyltransferase  definition   [KW-0328]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: P00492
Other
GeneWiki: Hypoxanthine-guanine_phosphoribosyltransferase
GenomeRNAi: 3251
PRO: PR:P00492