HPRT1 - Hypoxanthine-guanine phosphoribosyltransferase - human protein (Function)
 
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Protein
Gene
References

 
HPRT1 »  Hypoxanthine-guanine phosphoribosyltransferase   [ EC 2.4.2.8 ]  (HGPRT)
 
Gene name:  HPRT1
Entry whose protein(s) existence is based on evidence at protein level
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1 125 1
GENE REF ISO

Function

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Overview 
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.  
  • CuratedUniProtKB
GO molecular function 
Guanine phosphoribosyltransferase activitydefinition[GO:0052657]  
1
  • IDAUniProtKB
Hypoxanthine phosphoribosyltransferase activitydefinition[GO:0004422]  
3
  • IDAUniProtKB
Magnesium ion bindingdefinition[GO:0000287]  
1
  • IDAUniProtKB
Nucleotide bindingdefinition[GO:0000166]  
  • IEAUniProtKB KW
Protein bindingdefinition[GO:0005515]  
2
  • IPIIntAct
Protein homodimerization activitydefinition[GO:0042803]  
1
  • IPIUniProtKB
GO biological process 
Adenine salvagedefinition[GO:0006168] silver  
  • IBARefGenome
Central nervous system neuron developmentdefinition[GO:0021954] silver  
  • IEAOrtholog Compara
Cerebral cortex neuron differentiationdefinition[GO:0021895] silver  
  • IEAOrtholog Compara
Cytolysisdefinition[GO:0019835] silver  
  • IEAOrtholog Compara
Dendrite morphogenesisdefinition[GO:0048813] silver  
  • IEAOrtholog Compara
Dopamine metabolic processdefinition[GO:0042417] silver  
  • IEAOrtholog Compara
GMP catabolic processdefinition[GO:0046038]  
1
  • IDAUniProtKB
GMP salvagedefinition[GO:0032263] silver  
  • IBARefGenome
Grooming behaviordefinition[GO:0007625] silver  
  • IEAOrtholog Compara
Guanine salvagedefinition[GO:0006178]  
1
  • IDAUniProtKB
Hypoxanthine metabolic processdefinition[GO:0046100]  
1
  • IMPUniProtKB
Hypoxanthine salvagedefinition[GO:0043103]  
1
  • IDAUniProtKB
IMP metabolic processdefinition[GO:0046040]  
1
  • IDAUniProtKB
IMP salvagedefinition[GO:0032264]  
  • IBARefGenome
  • IEAUniPathway
Locomotory behaviordefinition[GO:0007626] silver  
  • IEAOrtholog Compara
Lymphocyte proliferationdefinition[GO:0046651] silver  
  • IEAOrtholog Compara
Positive regulation of dopamine metabolic processdefinition[GO:0045964]  
1
  • IMPUniProtKB
Protein homotetramerizationdefinition[GO:0051289]  
2
  • IPIUniProtKB
Purine nucleotide biosynthetic processdefinition[GO:0006164]  
1
  • IMPUniProtKB
Purine ribonucleoside salvagedefinition[GO:0006166]  
1
  • IMPUniProtKB
Response to amphetaminedefinition[GO:0001975] silver  
  • IEAOrtholog Compara
Striatum developmentdefinition[GO:0021756] silver  
  • IEAOrtholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
GMP + diphosphate guanine + 5-phospho-alpha-D-ribose 1-diphosphate.  
2
  • CuratedUniProtKB
EC 2.4.2.8: IMP + diphosphate hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.  
2
  • CuratedUniProtKB
It requires the following cofactor
Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.  
  • CuratedUniProtKB
Pathways 
This protein is involved in the following pathway
According to KEGG, this protein belongs to the following pathways:
Drug metabolism - other enzymes  hsa00983+3251  
Metabolic pathways  hsa01100+3251  
Purine metabolism  hsa00230+3251  
According to Reactome, this protein belongs to the following pathway:
Purine salvage  REACT_1923  
 
More information is available from:
 

Biophysicochemical properties

Kinetic parameters
KM 0.45 uM for hypoxanthine
KM 25 uM for pyrophosphate
KM 5.4 uM for IMP
KM 31 uM for phosphoribosylpyrophosphate

Keywords

Biological process 
Purine salvage  definition   [KW-0660]
Molecular function 
Glycosyltransferase  definition   [KW-0328]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: P00492
Other
GeneWiki: Hypoxanthine-guanine_phosphoribosyltransferase
GenomeRNAi: 3251
PRO: PR:P00492