GAPDH - Glyceraldehyde-3-phosphate dehydrogenase - human protein (Function)
 
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GAPDH »  Glyceraldehyde-3-phosphate dehydrogenase
 
Gene name:  GAPDH
Entry whose protein(s) existence is based on evidence at protein level
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1 185 2
GENE REF ISO

Function

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Overview 
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.  
3
  • CuratedUniProtKB
GO molecular function 
Glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activitydefinition[GO:0004365]  
2
  • NASUniProtKB
  • ISSOrtholog Curator
Identical protein bindingdefinition[GO:0042802]  
2
  • IPIIntAct
Microtubule bindingdefinition[GO:0008017]  
  • ISSOrtholog Curator
NAD bindingdefinition[GO:0051287] silver  
  • IEAInterPro 2 GO
NADP bindingdefinition[GO:0050661] silver  
  • IEAInterPro 2 GO
Peptidyl-cysteine S-nitrosylase activitydefinition[GO:0035605]  
  • ISSOrtholog Curator
Protein bindingdefinition[GO:0005515]  
8
  • IPIIntAct
  • IPIUniProtKB
GO biological process 
Cellular response to interferon-gammadefinition[GO:0071346]  
1
  • IDAUniProtKB
Glycolytic processdefinition[GO:0006096]  
  • IEAUniPathway
Microtubule cytoskeleton organizationdefinition[GO:0000226]  
  • ISSOrtholog Curator
Negative regulation of translationdefinition[GO:0017148]  
2
  • IDAUniProtKB
Neuron apoptotic processdefinition[GO:0051402]  
  • ISSOrtholog Curator
Oxidation-reduction processdefinition[GO:0055114]  
  • IEAUniProtKB KW
Peptidyl-cysteine S-trans-nitrosylationdefinition[GO:0035606]  
  • ISSOrtholog Curator
Protein stabilizationdefinition[GO:0050821]  
  • ISSOrtholog Curator
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 1.2.1.12: D-glyceraldehyde 3-phosphate + phosphate + NAD(+) 3-phospho-D-glyceroyl phosphate + NADH.  
1
  • CuratedUniProtKB
 
More information is available from:
Pathways 
This protein is involved in the following pathway
According to KEGG, this protein belongs to the following pathways:
Alzheimer's disease  hsa05010+2597  
Glycolysis / Gluconeogenesis  hsa00010+2597  
Metabolic pathways  hsa01100+2597  
According to Reactome, this protein belongs to the following pathways:
Gluconeogenesis  REACT_1520  
Glycolysis  REACT_1383  
 
More information is available from:
 

Keywords

Biological process 
Apoptosis  definition   [KW-0053]
Glycolysis  definition   [KW-0324]
Translation regulation  definition   [KW-0810]
Molecular function 
Oxidoreductase  definition   [KW-0560]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: P04406
Other
GeneWiki: Glyceraldehyde_3-phosphate_dehydrogenase
GenomeRNAi: 2597
PRO: PR:P04406