EPRS - Bifunctional glutamate/proline--tRNA ligase - human protein (Function)
 
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Protein
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References

 
EPRS »  Bifunctional glutamate/proline--tRNA ligase
 
Protein also known as:  Cell proliferation-inducing gene 32 protein.
Gene name:  EPRS
Family names: In the N-terminal section Class-I aminoacyl-tRNA synthetase
In the C-terminal section Class-II aminoacyl-tRNA synthetase
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 87 1
GENE REF ISO

Function

 show evidences
Overview 
Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.  
3
  • CuratedUniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
Protein bindingdefinition[GO:0005515]  
2
  • IPIIntAct
RNA stem-loop bindingdefinition[GO:0035613]  
1
  • IDAUniProtKB
GO biological process 
Cellular response to interferon-gammadefinition[GO:0071346]  
1
  • IDAUniProtKB
Glutamyl-tRNA aminoacylationdefinition[GO:0006424] silver  
  • IEAInterPro 2 GO
Negative regulation of translationdefinition[GO:0017148]  
2
  • IDAUniProtKB
Prolyl-tRNA aminoacylationdefinition[GO:0006433] silver  
  • IEAInterPro 2 GO
Protein complex assemblydefinition[GO:0006461]  
1
  • TASPINC
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC 6.1.1.17: ATP + L-glutamate + tRNA(Glu) AMP + diphosphate + L-glutamyl-tRNA(Glu).  
  • CuratedUniProtKB
EC 6.1.1.15: ATP + L-proline + tRNA(Pro) AMP + diphosphate + L-prolyl-tRNA(Pro).  
  • CuratedUniProtKB
Pathways 
According to KEGG, this protein belongs to the following pathways:
Aminoacyl-tRNA biosynthesis  hsa00970+2058  
Metabolic pathways  hsa01100+2058  
Porphyrin and chlorophyll metabolism  hsa00860+2058  
According to Reactome, this protein belongs to the following pathway:
Cytosolic tRNA aminoacylation  REACT_15306  
Caution 
Was originally thought to be a glutaminyl-tRNA synthetase.  
  • CuratedUniProtKB
 

Keywords

Biological process 
Protein biosynthesis  definition   [KW-0648]
Translation regulation  definition   [KW-0810]
Molecular function 
Aminoacyl-tRNA synthetase  definition   [KW-0030]
Ligase  definition   [KW-0436]
Technical term 
Multifunctional enzyme  definition   [KW-0511]
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: EPRS
GenomeRNAi: 2058
PRO: PR:P07814