UCHL3 - Ubiquitin carboxyl-terminal hydrolase isozyme L3 - human protein (Function)
 
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UCHL3 »  Ubiquitin carboxyl-terminal hydrolase isozyme L3   [ EC 3.4.19.12 ]  (UCH-L3)
 
Protein also known as:  Ubiquitin thioesterase L3.
Gene name:  UCHL3
Family name: Peptidase C12
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.  
5
  • CuratedUniProtKB
GO molecular function 
Cysteine-type peptidase activitydefinition[GO:0008234]  
  • IEAUniProtKB KW
Peptidase activitydefinition[GO:0008233]  
1
  • IDAUniProtKB
Protein bindingdefinition[GO:0005515]  
1
  • IPIIntAct
Ubiquitin bindingdefinition[GO:0043130]  
1
  • IDAUniProtKB
Ubiquitin thiolesterase activitydefinition[GO:0004221]  
1
  • TASUniProtKB
GO biological process 
Protein catabolic processdefinition[GO:0030163]  
1
  • IDAUniProtKB
Ubiquitin-dependent protein catabolic processdefinition[GO:0006511] silver  
  • IEAInterPro 2 GO
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.4.19.12: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).  
3
  • CuratedUniProtKB
It is regulated in the following manner
Inhibited by monoubiquitin and diubiquitin.  
1
  • CuratedUniProtKB
 
More information is available from:
 

Keywords

Biological process 
Ubl conjugation pathway  definition   [KW-0833]
Molecular function 
Hydrolase  definition   [KW-0378]
Protease  definition   [KW-0645]
Thiol protease  definition   [KW-0788]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: P15374
Other
GeneWiki: UCHL3
GenomeRNAi: 7347
PRO: PR:P15374