ALOX15 - Arachidonate 15-lipoxygenase - human protein (Function)
 
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ALOX15 »  Arachidonate 15-lipoxygenase   [ EC 1.13.11.33 ]  (15-LOX)
 
Protein also known as:  Arachidonate 12-lipoxygenase, leukocyte-type [EC 1.13.11.31] (12-LOX).
Gene name:  ALOX15
Family name: Lipoxygenase
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 125 2
GENE REF ISO

Function

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Overview 
Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass.  
3
  • UniProtKB
GO molecular function 
Arachidonate 12-lipoxygenase activitydefinition[GO:0004052]  
1
  • UniProtKB
Arachidonate 15-lipoxygenase activitydefinition[GO:0050473]  
3
  • UniProtKB
Hepoxilin A3 synthase activitydefinition[GO:0051120] silver  
1
  • Ortholog Compara
Hepoxilin-epoxide hydrolase activitydefinition[GO:0047977] silver  
1
  • Ortholog Compara
Iron ion bindingdefinition[GO:0005506]  
1
  • Ortholog Curator
Phosphatidylinositol-4,5-bisphosphate bindingdefinition[GO:0005546]  
1
  • UniProtKB
Protein bindingdefinition[GO:0005515]  
1
  • UniProtKB
GO biological process 
Apoptotic cell clearancedefinition[GO:0043277]  
1
  • Ortholog Curator
Arachidonic acid metabolic processdefinition[GO:0019369]  
3
  • UniProtKB
Bone mineralizationdefinition[GO:0030282]  
1
  • Ortholog Curator
Cellular response to calcium iondefinition[GO:0071277]  
1
  • UniProtKB
Cellular response to interleukin-13definition[GO:0035963]  
1
  • UniProtKB
Hepoxilin biosynthetic processdefinition[GO:0051122]  
1
  • Ortholog Curator
Inflammatory responsedefinition[GO:0006954]  
1
  • PINC
Lipoxin A4 biosynthetic processdefinition[GO:2001303]  
1
  • Ortholog Curator
Lipoxygenase pathwaydefinition[GO:0019372]  
1
  • UniProtKB
Negative regulation of adaptive immune responsedefinition[GO:0002820]  
1
  • Ortholog Curator
Ossificationdefinition[GO:0001503]  
1
  • Ortholog Curator
Oxidation-reduction processdefinition[GO:0055114]  
1
  • UniProtKB KW
Phosphatidylethanolamine biosynthetic processdefinition[GO:0006646]  
1
  • Ortholog Curator
Positive regulation of actin filament polymerizationdefinition[GO:0030838]  
1
  • Ortholog Curator
Positive regulation of cell-substrate adhesiondefinition[GO:0010811]  
1
  • UniProtKB
Positive regulation of ERK1 and ERK2 cascadedefinition[GO:0070374]  
1
  • UniProtKB
Positive regulation of heterotypic cell-cell adhesiondefinition[GO:0034116] silver  
1
  • Ortholog Compara
Regulation of engulfment of apoptotic celldefinition[GO:1901074]  
1
  • Ortholog Curator
Regulation of peroxisome proliferator activated receptor signaling pathwaydefinition[GO:0035358]  
1
  • Ortholog Curator
Response to endoplasmic reticulum stressdefinition[GO:0034976]  
1
  • Ortholog Curator
Wound healingdefinition[GO:0042060]  
1
  • Ortholog Curator
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC 1.13.11.31: Arachidonate + O(2) (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.  
1
  • UniProtKB
EC 1.13.11.33: Arachidonate + O(2) (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate.  
1
  • UniProtKB
It requires the following cofactor
 Fe cation : Binds 1 Fe cation per subunit.  
2
  • PROSITE-ProRule
  • UniProtKB
It is regulated in the following manner
Activity is increased by binding phosphatidylinositol phosphates, especially phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate.  
2
  • UniProtKB
Pathways 
This protein is involved in the following pathway
Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis  
1
  • UniProtKB
According to KEGG, this protein belongs to the following pathways:
Arachidonic acid metabolism  hsa00590+246  
Linoleic acid metabolism  hsa00591+246  
Metabolic pathways  hsa01100+246  
According to Reactome, this protein belongs to the following pathways:
Synthesis of 12-eicosatetraenoic acid derivatives  REACT_150201  
Synthesis of 15-eicosatetraenoic acid derivatives  REACT_150422  
Synthesis of Leukotrienes (LT) and Eoxins (EX)  REACT_150420  
 
More information is available from:
 

Keywords

Biological process 
Fatty acid metabolism  definition   [KW-0276]
Lipid metabolism  definition   [KW-0443]
Molecular function 
Dioxygenase  definition   [KW-0223]
Oxidoreductase  definition   [KW-0560]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: ALOX15
GenomeRNAi: 246
PRO: PR:P16050