PAM - Peptidyl-glycine alpha-amidating monooxygenase - human protein (Function)
 
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PAM »  Peptidyl-glycine alpha-amidating monooxygenase  (PAM)
 
Gene name:  PAM
Family names: In the N-terminal section Copper type II ascorbate-dependent monooxygenase
In the C-terminal section Peptidyl-alpha-hydroxyglycine alpha-amidating lyase
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.  
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  • UniProtKB
GO molecular function 
Copper ion bindingdefinition[GO:0005507] silver  
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  • InterPro 2 GO
L-ascorbic acid bindingdefinition[GO:0031418]  
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  • UniProtKB KW
Peptidylamidoglycolate lyase activitydefinition[GO:0004598]  
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  • UniProtKB
Peptidylglycine monooxygenase activitydefinition[GO:0004504]  
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  • UniProtKB
Protein bindingdefinition[GO:0005515]  
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  • UniProtKB
Zinc ion bindingdefinition[GO:0008270]  
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  • UniProtKB
GO biological process 
Oxidation-reduction processdefinition[GO:0055114]  
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  • UniProtKB KW
Peptide amidationdefinition[GO:0001519]  
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  • UniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC 1.14.17.3: Peptidylglycine + ascorbate + O(2) peptidyl(2-hydroxyglycine) + dehydroascorbate + H(2)O.  
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  • UniProtKB
EC 4.3.2.5: Peptidylamidoglycolate peptidyl amide + glyoxylate.  
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  • UniProtKB
It requires the following cofactors
 Zn(2+) : Zn(2+) is required for the lyase reaction.  
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  • UniProtKB
 Cu(2+) : Binds 2 copper ions per subunit for the monoxygenase reaction.  
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  • UniProtKB
It is regulated in the following manner
Inhibited by EDTA, phenylglyoxal and diethyl pyrocarbonate.  
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  • UniProtKB
 

Keywords

Molecular function 
Lyase  definition   [KW-0456]
Monooxygenase  definition   [KW-0503]
Oxidoreductase  definition   [KW-0560]
Technical term 
Multifunctional enzyme  definition   [KW-0511]
Reference proteome  definition   [KW-1185]
 

Further external links