PAM - Peptidyl-glycine alpha-amidating monooxygenase - human protein (Function)
 
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PAM »  Peptidyl-glycine alpha-amidating monooxygenase  (PAM)
 
Gene name:  PAM
Family names: In the N-terminal section Copper type II ascorbate-dependent monooxygenase
In the C-terminal section Peptidyl-alpha-hydroxyglycine alpha-amidating lyase
Entry whose protein(s) existence is based on evidence at protein level
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1 45 6
GENE REF ISO

Function

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Overview 
Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.  
  • CuratedUniProtKB
GO molecular function 
Calcium ion bindingdefinition[GO:0005509] silver  
  • IEAOrtholog Compara
Copper ion bindingdefinition[GO:0005507] silver  
  • IEAInterPro 2 GO
L-ascorbic acid bindingdefinition[GO:0031418]  
  • IEAUniProtKB KW
Peptidylamidoglycolate lyase activitydefinition[GO:0004598]  
1
  • IDAUniProtKB
Peptidylglycine monooxygenase activitydefinition[GO:0004504]  
2
  • IDAUniProtKB
Protein bindingdefinition[GO:0005515]  
1
  • IPIUniProtKB
Protein kinase bindingdefinition[GO:0019901] silver  
  • IEAOrtholog Compara
Zinc ion bindingdefinition[GO:0008270]  
1
  • IDAUniProtKB
GO biological process 
Central nervous system developmentdefinition[GO:0007417] silver  
  • IEAOrtholog Compara
Heart developmentdefinition[GO:0007507] silver  
  • IEAOrtholog Compara
Lactationdefinition[GO:0007595] silver  
  • IEAOrtholog Compara
Limb developmentdefinition[GO:0060173] silver  
  • IEAOrtholog Compara
Long-chain fatty acid metabolic processdefinition[GO:0001676] silver  
  • IEAOrtholog Compara
Maternal process involved in female pregnancydefinition[GO:0060135] silver  
  • IEAOrtholog Compara
Odontogenesisdefinition[GO:0042476] silver  
  • IEAOrtholog Compara
Ovulation cycle processdefinition[GO:0022602] silver  
  • IEAOrtholog Compara
Oxidation-reduction processdefinition[GO:0055114]  
  • IEAUniProtKB KW
Peptide amidationdefinition[GO:0001519]  
2
  • IDAUniProtKB
Protein amidationdefinition[GO:0018032] silver  
  • IEAOrtholog Compara
Protein homooligomerizationdefinition[GO:0051260] silver  
  • IEAOrtholog Compara
Regulation of actin cytoskeleton organizationdefinition[GO:0032956] silver  
  • IEAOrtholog Compara
Regulation of protein secretiondefinition[GO:0050708] silver  
  • IEAOrtholog Compara
Regulation of transcription from RNA polymerase II promoterdefinition[GO:0006357] silver  
  • IEAOrtholog Compara
Response to copper iondefinition[GO:0046688] silver  
  • IEAOrtholog Compara
Response to drugdefinition[GO:0042493] silver  
  • IEAOrtholog Compara
Response to estradioldefinition[GO:0032355] silver  
  • IEAOrtholog Compara
Response to glucocorticoiddefinition[GO:0051384] silver  
  • IEAOrtholog Compara
Response to hypoxiadefinition[GO:0001666] silver  
  • IEAOrtholog Compara
Response to pHdefinition[GO:0009268] silver  
  • IEAOrtholog Compara
Toxin metabolic processdefinition[GO:0009404] silver  
  • IEAOrtholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC 1.14.17.3: Peptidylglycine + ascorbate + O(2) peptidyl(2-hydroxyglycine) + dehydroascorbate + H(2)O.  
  • CuratedUniProtKB
EC 4.3.2.5: Peptidylamidoglycolate peptidyl amide + glyoxylate.  
  • CuratedUniProtKB
It requires the following cofactors
Zinc; for the lyase reaction.  
1
  • CuratedUniProtKB
Binds 2 copper ions per subunit; For the monoxygenase reaction.  
1
  • CuratedUniProtKB
It is regulated in the following manner
Inhibited by EDTA, phenylglyoxal and diethyl pyrocarbonate.  
1
  • CuratedUniProtKB
 

Keywords

Molecular function 
Lyase  definition   [KW-0456]
Monooxygenase  definition   [KW-0503]
Oxidoreductase  definition   [KW-0560]
Technical term 
Multifunctional enzyme  definition   [KW-0511]
Reference proteome  definition   [KW-1185]
 

Further external links