PRKCH - Protein kinase C eta type - human protein (Function)
 
Home Recent activites arrow-down favorite My favorites arrow-down favorite My labels arrow-down Downloads
Back to ...  
Publication View
Protein
Gene
References

 
PRKCH »  Protein kinase C eta type
 
Gene name:  PRKCH
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 74 1
GENE REF ISO

Function

 show evidences
Overview 
Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.  
10
  • CuratedUniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
Calcium-independent protein kinase C activitydefinition[GO:0004699] silver  
  • IEAOrtholog Compara
Enzyme bindingdefinition[GO:0019899]  
1
  • IPIUniProtKB
Metal ion bindingdefinition[GO:0046872]  
  • IEAUniProtKB KW
Protein kinase C activitydefinition[GO:0004697]  
1
  • TASPINC
Ral GTPase bindingdefinition[GO:0017160] silver  
  • IEAOrtholog Compara
GO biological process 
Negative regulation of glial cell apoptotic processdefinition[GO:0034351]  
1
  • IMPUniProtKB
Positive regulation of B cell receptor signaling pathwaydefinition[GO:0050861]  
1
  • IMPUniProtKB
Positive regulation of glial cell proliferationdefinition[GO:0060252]  
1
  • IMPUniProtKB
Positive regulation of keratinocyte differentiationdefinition[GO:0045618]  
  • ISSOrtholog Curator
Positive regulation of macrophage derived foam cell differentiationdefinition[GO:0010744]  
  • ISSOrtholog Curator
Positive regulation of NF-kappaB transcription factor activitydefinition[GO:0051092]  
1
  • IMPUniProtKB
Protein kinase C signalingdefinition[GO:0070528] silver  
  • IEAOrtholog Compara
Protein phosphorylationdefinition[GO:0006468]  
1
  • IMPUniProtKB
Regulation of tight junction assemblydefinition[GO:2000810]  
1
  • IMPUniProtKB
Signal transductiondefinition[GO:0007165]  
1
  • TASPINC
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.7.11.13: ATP + a protein ADP + a phosphoprotein.  
  • CuratedUniProtKB
It is regulated in the following manner
Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation.  
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathways:
Tight junction  hsa04530+5583  
Vascular smooth muscle contraction  hsa04270+5583  
According to Reactome, this protein belongs to the following pathways:
Effects of PIP2 hydrolysis  REACT_2202  
G alpha (z) signalling events  REACT_19333  
 

Keywords

Biological process 
Differentiation  definition   [KW-0221]
Molecular function 
Kinase  definition   [KW-0418]
Serine/threonine-protein kinase  definition   [KW-0723]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Other
GeneWiki: PRKCH
GenomeRNAi: 5583
PRO: PR:P24723
Chemistry
GuidetoPHARMACOLOGY: 1487