NOS2 - Nitric oxide synthase, inducible - human protein (Function)
 
Home Recent activites arrow-down favorite My favorites arrow-down favorite My labels arrow-down Downloads
Back to ...  
Publication View
Protein
Gene
References

 
NOS2 »  Nitric oxide synthase, inducible   [ EC 1.14.13.39 ]
 
Protein also known as:  Peptidyl-cysteine S-nitrosylase NOS2.
Gene name:  NOS2
Family name: NOS
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 78 2
GENE REF ISO

Function

 show evidences
Overview 
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.  
  • CuratedUniProtKB
GO molecular function 
Arginine bindingdefinition[GO:0034618]  
  • ISSOrtholog Curator
Calmodulin bindingdefinition[GO:0005516]  
  • IEAUniProtKB KW
Flavin adenine dinucleotide bindingdefinition[GO:0050660]  
  • ISSOrtholog Curator
FMN bindingdefinition[GO:0010181]  
  • ISSOrtholog Curator
Heme bindingdefinition[GO:0020037]  
  • ISSOrtholog Curator
Iron ion bindingdefinition[GO:0005506] silver  
  • IEAInterPro 2 GO
NADP bindingdefinition[GO:0050661]  
1
  • TASBHF-UCL
NADPH-hemoprotein reductase activitydefinition[GO:0003958] silver  
  • IBARefGenome
Nitric-oxide synthase activitydefinition[GO:0004517]  
2
  • IDABHF-UCL
  • IDAUniProtKB
Protein bindingdefinition[GO:0005515]  
1
  • IPIUniProtKB
Protein homodimerization activitydefinition[GO:0042803]  
  • ISSOrtholog Curator
Receptor bindingdefinition[GO:0005102]  
1
  • IPIUniProtKB
Tetrahydrobiopterin bindingdefinition[GO:0034617]  
  • ISSOrtholog Curator
GO biological process 
Arginine catabolic processdefinition[GO:0006527]  
1
  • IDABHF-UCL
Cellular response to interferon-gammadefinition[GO:0071346] silver  
  • IEAOrtholog Compara
Cellular response to lipopolysaccharidedefinition[GO:0071222] silver  
  • IEAOrtholog Compara
Defense response to bacteriumdefinition[GO:0042742]  
1
  • IMPBHF-UCL
  • ISSOrtholog Curator
Defense response to Gram-negative bacteriumdefinition[GO:0050829]  
1
  • NASBHF-UCL
Inflammatory responsedefinition[GO:0006954] silver  
  • IBARefGenome
Innate immune response in mucosadefinition[GO:0002227]  
1
  • NASBHF-UCL
Negative regulation of blood pressuredefinition[GO:0045776] silver  
  • IBARefGenome
Negative regulation of gene expressiondefinition[GO:0010629]  
1
  • IGIUniProtKB
Negative regulation of protein catabolic processdefinition[GO:0042177] silver  
  • IEAOrtholog Compara
Nitric oxide biosynthetic processdefinition[GO:0006809]  
2
  • IDABHF-UCL
  • IDAUniProtKB
Nitric oxide mediated signal transductiondefinition[GO:0007263] silver  
  • IBARefGenome
Oxidation-reduction processdefinition[GO:0055114]  
  • IEAUniProtKB KW
Peptidyl-cysteine S-nitrosylationdefinition[GO:0018119]  
  • ISSOrtholog Curator
Positive regulation of guanylate cyclase activitydefinition[GO:0031284] silver  
  • IBARefGenome
Positive regulation of killing of cells of other organismdefinition[GO:0051712]  
1
  • IMPBHF-UCL
Positive regulation of leukocyte mediated cytotoxicitydefinition[GO:0001912]  
1
  • TASBHF-UCL
Positive regulation of vasodilationdefinition[GO:0045909] silver  
  • IBARefGenome
Regulation of cell proliferationdefinition[GO:0042127] silver  
  • IEAOrtholog Compara
Regulation of cellular respirationdefinition[GO:0043457]  
1
  • TASBHF-UCL
Regulation of insulin secretiondefinition[GO:0050796]  
1
  • IMPBHF-UCL
Response to bacteriumdefinition[GO:0009617]  
1
  • NASUniProtKB
Response to hypoxiadefinition[GO:0001666] silver  
  • IEAOrtholog Compara
Superoxide metabolic processdefinition[GO:0006801]  
  • ISSOrtholog Curator
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 1.14.13.39: 2 L-arginine + 3 NADPH + 4 O(2) 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.  
  • CuratedUniProtKB
It requires the following cofactors
Heme group (By similarity).  
  • CuratedUniProtKB
Binds 1 FAD (By similarity).  
  • CuratedUniProtKB
Binds 1 FMN (By similarity).  
  • CuratedUniProtKB
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).  
  • CuratedUniProtKB
It is regulated in the following manner
Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity (By similarity).  
  • CuratedUniProtKB
Pathways 
According to KEGG, this protein belongs to the following pathways:
Amoebiasis  hsa05146+4843  
Arginine and proline metabolism  hsa00330+4843  
Calcium signaling pathway  hsa04020+4843  
Chagas disease (American trypanosomiasis)  hsa05142+4843  
Leishmaniasis  hsa05140+4843  
Metabolic pathways  hsa01100+4843  
Pathways in cancer  hsa05200+4843  
Peroxisome  hsa04146+4843  
Pertussis  hsa05133+4843  
Salmonella infection  hsa05132+4843  
Small cell lung cancer  hsa05222+4843  
Toxoplasmosis  hsa05145+4843  
Tuberculosis  hsa05152+4843  
According to Reactome, this protein belongs to the following pathways:
Nitric oxide stimulates guanylate cyclase  REACT_23862  
Phagosomal maturation (early endosomal stage)  REACT_121256  
 
More information is available from:
 

Keywords

Molecular function 
Oxidoreductase  definition   [KW-0560]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Other
GeneWiki: Nitric_oxide_synthase_2_(inducible)
GenomeRNAi: 4843
PRO: PR:P35228
Chemistry
GuidetoPHARMACOLOGY: 1250