CDK8 - Cyclin-dependent kinase 8 - human protein (Function)
 
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CDK8 »  Cyclin-dependent kinase 8   [ EC 2.7.11.22EC 2.7.11.23 ]
 
Protein also known as:  Mediator of RNA polymerase II transcription subunit CDK8.
Gene name:  CDK8
Entry whose protein(s) existence is based on evidence at protein level
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1 55 2
GENE REF ISO

Function

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Overview 
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.  
2
  • CuratedUniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
Cyclin-dependent protein serine/threonine kinase activitydefinition[GO:0004693]  
  • IEAEC 2 GO
Protein bindingdefinition[GO:0005515]  
6
  • IPIIntAct
  • IPIUniProtKB
Protein kinase activitydefinition[GO:0004672]  
1
  • TASPINC
RNA polymerase II carboxy-terminal domain kinase activitydefinition[GO:0008353]  
  • IEAEC 2 GO
GO biological process 
Protein phosphorylationdefinition[GO:0006468] silver  
  • IEAInterPro 2 GO
Regulation of cell cycledefinition[GO:0051726]  
  • IEAEC 2 GO
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC 2.7.11.22: ATP + a protein ADP + a phosphoprotein.  
1
  • CuratedUniProtKB
EC 2.7.11.23: ATP + [DNA-directed RNA polymerase] ADP + [DNA-directed RNA polymerase] phosphate.  
1
  • CuratedUniProtKB
It requires the following cofactor
Magnesium (By similarity).  
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to Reactome, this protein belongs to the following pathways:
Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants  REACT_160254  
Constitutive Signaling by NOTCH1 PEST Domain Mutants  REACT_160243  
Generic Transcription Pathway  REACT_12627  
NOTCH1 Intracellular Domain Regulates Transcription  REACT_118780  
PPARA activates gene expression  REACT_116145  
SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription  REACT_120734  
Transcriptional regulation of white adipocyte differentiation  REACT_27161  
 

Keywords

Biological process 
Transcription  definition   [KW-0804]
Transcription regulation  definition   [KW-0805]
Molecular function 
Activator  definition   [KW-0010]
Kinase  definition   [KW-0418]
Repressor  definition   [KW-0678]
Serine/threonine-protein kinase  definition   [KW-0723]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Other
GeneWiki: Cyclin-dependent_kinase_8
GenomeRNAi: 1024
PRO: PR:P49336
Chemistry
GuidetoPHARMACOLOGY: 1980