CDC34 - Ubiquitin-conjugating enzyme E2 R1 - human protein (Function)
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CDC34 »  Ubiquitin-conjugating enzyme E2 R1   [ EC ]
Protein also known as:  Ubiquitin-conjugating enzyme E2-32 kDa complementing.
Gene name:  CDC34
Entry whose protein(s) existence is based on evidence at protein level
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Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.  
  • CuratedUniProtKB
GO molecular function 
Acid-amino acid ligase activitydefinition[GO:0016881] silver  
  • IEAInterPro 2 GO
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
Protein bindingdefinition[GO:0005515]  
  • IPIIntAct
  • IPIUniProtKB
Ubiquitin-protein transferase activitydefinition[GO:0004842]  
  • IDAUniProtKB
GO biological process 
Cellular protein modification processdefinition[GO:0006464]  
  • NASUniProtKB
DNA replication initiationdefinition[GO:0006270]  
  • NASUniProtKB
G1/S transition of mitotic cell cycledefinition[GO:0000082]  
  • NASUniProtKB
Negative regulation of cAMP-mediated signalingdefinition[GO:0043951]  
  • IDAUniProtKB
Positive regulation of inclusion body assemblydefinition[GO:0090261] silver  
  • IEAOrtholog Compara
Positive regulation of neuron apoptotic processdefinition[GO:0043525] silver  
  • IEAOrtholog Compara
Proteasome-mediated ubiquitin-dependent protein catabolic processdefinition[GO:0043161]  
  • IDAUniProtKB
Protein K48-linked ubiquitinationdefinition[GO:0070936]  
  • IDAUniProtKB
Protein polyubiquitinationdefinition[GO:0000209]  
  • IDAUniProtKB
Protein ubiquitinationdefinition[GO:0016567]  
  • IDAUniProtKB
Response to growth factordefinition[GO:0070848] silver  
  • IEAOrtholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC ATP + ubiquitin + protein lysine AMP + diphosphate + protein N-ubiquityllysine.  
  • CuratedUniProtKB
It is regulated in the following manner
CDC34-catalyzed polyubiquitin chain assembly activity is stimulated by the conjugation of NEDD8 to the CUL1 SCF E3 ligase complex subunit.  
  • CuratedUniProtKB
This protein is involved in the following pathway
According to KEGG, this protein belongs to the following pathways:
Herpes simplex infection  hsa05168+997  
Ubiquitin mediated proteolysis  hsa04120+997  
According to Reactome, this protein belongs to the following pathway:
Antigen processing: Ubiquitination & Proteasome degradation  REACT_75842  

Biophysicochemical properties

Kinetic parameters
KM 0.11 uM for beta-catenin-monoubiquin


Biological process 
Cell cycle  definition   [KW-0131]
Ubl conjugation pathway  definition   [KW-0833]
Molecular function 
Ligase  definition   [KW-0436]
Technical term 
Reference proteome  definition   [KW-1185]

Further external links

GeneWiki: CDC34
GenomeRNAi: 997
PRO: PR:P49427