ACOT2 - Acyl-coenzyme A thioesterase 2, mitochondrial - human protein (Function)
 
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ACOT2 »  Acyl-coenzyme A thioesterase 2, mitochondrial   [ EC 3.1.2.2 ]  (Acyl-CoA thioesterase 2)
 
Protein also known as:  Acyl-coenzyme A thioester hydrolase 2a.
Gene name:  ACOT2
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 16 2
GENE REF ISO

Function

 show evidences
Overview 
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs.  
1
  • CuratedUniProtKB
GO molecular function 
Acyl-CoA hydrolase activitydefinition[GO:0047617]  
2
  • IDAHGNC
Palmitoyl-CoA hydrolase activitydefinition[GO:0016290]  
  • IEAEC 2 GO
Receptor bindingdefinition[GO:0005102]  
1
  • IPIUniProtKB
GO biological process 
Acyl-CoA metabolic processdefinition[GO:0006637]  
2
  • IDAHGNC
Long-chain fatty acid metabolic processdefinition[GO:0001676]  
1
  • IDAHGNC
Very long-chain fatty acid metabolic processdefinition[GO:0000038]  
1
  • IDAHGNC
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.1.2.2: Palmitoyl-CoA + H(2)O CoA + palmitate.  
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathways:
Biosynthesis of unsaturated fatty acids  hsa01040+10965  
Fatty acid elongation  hsa00062+10965  
 
More information is available from:
Caution 
Was originally (PubMed10944470) thought to be peroxisomal but was later shown (PubMed16940157) to be mitochondrial.  
2
  • CuratedUniProtKB
 

Biophysicochemical properties

Kinetic parameters
KM 6.1 uM for C18:1-acyl-CoA
KM 4.8 uM for C20-acyl-CoA
KM 8.9 uM for C12-acyl-CoA
KM 2.0 uM for C16-acyl-CoA
KM 40.3 uM for C10-acyl-CoA
KM 4.3 uM for C18:1-trans-acyl-CoA
KM 4.5 uM for C16:1-acyl-CoA
KM 2.8 uM for C18-acyl-CoA
KM 1.6 uM for C14-acyl-CoA
Vmax 488 nmol/min/mg enzyme toward C18-acyl-CoA
Vmax 661 nmol/min/mg enzyme toward C16:1-acyl-CoA
Vmax 408 nmol/min/mg enzyme toward C20-acyl-CoA
Vmax 656 nmol/min/mg enzyme toward C16-acyl-CoA
Vmax 418 nmol/min/mg enzyme toward C18:1-trans-acyl-CoA
Vmax 681 nmol/min/mg enzyme toward C12-acyl-CoA
Vmax 766 nmol/min/mg enzyme toward C14-acyl-CoA
Vmax 304 nmol/min/mg enzyme toward C18:1-acyl-CoA
Vmax 212 nmol/min/mg enzyme toward C10-acyl-CoA

Keywords

Molecular function 
Hydrolase  definition   [KW-0378]
Serine esterase  definition   [KW-0719]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: P49753
Other
GeneWiki: ACOT2
GenomeRNAi: 10965
PRO: PR:P49753