CDK7 - Cyclin-dependent kinase 7 - human protein (Function)
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CDK7 »  Cyclin-dependent kinase 7   [ EC ]
Protein also known as:  TFIIH basal transcription factor complex kinase subunit.
Gene name:  CDK7
Entry whose protein(s) existence is based on evidence at protein level
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Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.  
  • UniProtKB
GO molecular function 
Androgen receptor bindingdefinition[GO:0050681]  
  • UniProtKB
ATP bindingdefinition[GO:0005524]  
  • UniProtKB KW
Cyclin-dependent protein serine/threonine kinase activitydefinition[GO:0004693]  
  • EC 2 GO
DNA-dependent ATPase activitydefinition[GO:0008094]  
  • UniProtKB
Protein bindingdefinition[GO:0005515]  
  • IntAct
Protein C-terminus bindingdefinition[GO:0008022]  
  • UniProtKB
Protein kinase activitydefinition[GO:0004672]  
  • PINC
RNA polymerase II carboxy-terminal domain kinase activitydefinition[GO:0008353]  
  • UniProtKB
Transcription coactivator activitydefinition[GO:0003713]  
  • UniProtKB
GO biological process 
Androgen receptor signaling pathwaydefinition[GO:0030521]  
  • UniProtKB
Cell cycle arrestdefinition[GO:0007050]  
  • UniProtKB
Cell divisiondefinition[GO:0051301]  
  • UniProtKB KW
Cell proliferationdefinition[GO:0008283]  
  • PINC
Positive regulation of transcription from RNA polymerase II promoterdefinition[GO:0045944]  
  • UniProtKB
Positive regulation of transcription, DNA-templateddefinition[GO:0045893]  
  • UniProtKB
Protein phosphorylationdefinition[GO:0006468] silver  
  • InterPro 2 GO
Regulation of cyclin-dependent protein serine/threonine kinase activitydefinition[GO:0000079]  
  • PINC
Transcription from RNA polymerase II promoterdefinition[GO:0006366]  
  • UniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC ATP + a protein ADP + a phosphoprotein.  
  • UniProtKB
EC ATP + [DNA-directed RNA polymerase] ADP + [DNA-directed RNA polymerase] phosphate.  
  • UniProtKB
It is regulated in the following manner
Inactivated by phosphorylation. Repressed by roscovitine (seliciclib, CYC202), R547 (Ro-4584820) and SNS-032 (BMS-387032). The association of p53/TP53 to the CAK complex in response to DNA damage reduces kinase activity toward CDK2 and RNA polymerase II repetitive C-terminal domain (CTD), thus stopping cell cycle progression. The inactivation by roscovitine promotes caspase-mediated apoptosis in leukemic cells.  
  • UniProtKB
More information is available from:
According to KEGG, this protein belongs to the following pathways:
Basal transcription factors  hsa03022+1022  
Cell cycle  hsa04110+1022  
Nucleotide excision repair  hsa03420+1022  
According to Reactome, this protein belongs to the following pathways:
Cyclin A/B1 associated events during G2/M transition  R-HSA-69273  
Cyclin A:Cdk2-associated events at S phase entry  R-HSA-69656  
Cyclin D associated events in G1  R-HSA-69231  
Cyclin E associated events during G1/S transition  R-HSA-69202  
Dual incision in TC-NER  R-HSA-6782135  
Formation of HIV elongation complex in the absence of HIV Tat  R-HSA-167152  
Formation of HIV-1 elongation complex containing HIV-1 Tat  R-HSA-167200  
Formation of Incision Complex in GG-NER  R-HSA-5696395  
Formation of RNA Pol II elongation complex  R-HSA-112382  
Formation of TC-NER Pre-Incision Complex  R-HSA-6781823  
Formation of the Early Elongation Complex  R-HSA-113418  
Formation of the HIV-1 Early Elongation Complex  R-HSA-167158  
Gap-filling DNA repair synthesis and ligation in TC-NER  R-HSA-6782210  
HIV Transcription Initiation  R-HSA-167161  
mRNA Capping  R-HSA-72086  
NoRC negatively regulates rRNA expression  R-HSA-427413  
RNA Pol II CTD phosphorylation and interaction with CE  R-HSA-167160  
RNA Pol II CTD phosphorylation and interaction with CE  R-HSA-77075  
RNA Polymerase I Chain Elongation  R-HSA-73777  
RNA Polymerase I Promoter Escape  R-HSA-73772  
RNA Polymerase I Transcription Initiation  R-HSA-73762  
RNA Polymerase I Transcription Termination  R-HSA-73863  
RNA Polymerase II HIV Promoter Escape  R-HSA-167162  
RNA Polymerase II Pre-transcription Events  R-HSA-674695  
RNA Polymerase II Promoter Escape  R-HSA-73776  
RNA Polymerase II Transcription Elongation  R-HSA-75955  
RNA Polymerase II Transcription Initiation  R-HSA-75953  
RNA Polymerase II Transcription Initiation And Promoter Clearance  R-HSA-76042  
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening  R-HSA-73779  
Tat-mediated elongation of the HIV-1 transcript  R-HSA-167246  
Transcription of the HIV genome  R-HSA-167172  
Transcription-Coupled Nucleotide Excision Repair (TC-NER)  R-HSA-6781827  


Biological process 
Cell cycle  definition   [KW-0131]
Cell division  definition   [KW-0132]
DNA damage  definition   [KW-0227]
DNA repair  definition   [KW-0234]
Transcription  definition   [KW-0804]
Transcription regulation  definition   [KW-0805]
Molecular function 
Kinase  definition   [KW-0418]
Serine/threonine-protein kinase  definition   [KW-0723]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]

Further external links

GeneWiki: Cyclin-dependent_kinase_7
GenomeRNAi: 1022
PRO: PR:P50613