PRKX - cAMP-dependent protein kinase catalytic subunit PRKX - human protein (Function)
 
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PRKX »  cAMP-dependent protein kinase catalytic subunit PRKX   [ EC 2.7.11.1 ]  (PrKX)
 
Protein also known as:  Protein kinase PKX1.
Gene name:  PRKX
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.  
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  • CuratedUniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
cAMP-dependent protein kinase activitydefinition[GO:0004691]  
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  • IDAUniProtKB
Protein bindingdefinition[GO:0005515]  
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  • IPIIntAct
  • IPIUniProtKB
GO biological process 
Angiogenesisdefinition[GO:0001525]  
1
  • IMPUniProtKB
Cell adhesiondefinition[GO:0007155]  
1
  • IMPUniProtKB
Cell-substrate adhesiondefinition[GO:0031589]  
1
  • IMPUniProtKB
Endothelial cell migrationdefinition[GO:0043542]  
1
  • IMPUniProtKB
Endothelial cell proliferationdefinition[GO:0001935]  
1
  • IMPUniProtKB
Epithelial tube morphogenesisdefinition[GO:0060562]  
1
  • IDAUniProtKB
Kidney morphogenesisdefinition[GO:0060993]  
1
  • IDAUniProtKB
Myeloid cell differentiationdefinition[GO:0030099]  
1
  • IDAUniProtKB
Peptidyl-serine phosphorylationdefinition[GO:0018105]  
1
  • IDAUniProtKB
Protein autophosphorylationdefinition[GO:0046777]  
2
  • IDAUniProtKB
Regulation of cell adhesiondefinition[GO:0030155]  
1
  • IDAUniProtKB
Regulation of cell migrationdefinition[GO:0030334]  
1
  • IDAUniProtKB
Regulation of epithelial cell differentiation involved in kidney developmentdefinition[GO:2000696]  
1
  • IDAUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.7.11.1: ATP + a protein ADP + a phosphoprotein.  
2
  • CuratedUniProtKB
It is regulated in the following manner
Binding of cAMP to the PRKAR1A or PRKAR1B regulatory subunits induces dissociation of the holoenzyme heterotetramer. The released monomeric PRKX is then active and able to phosphorylate its substrates.  
3
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathways:
Apoptosis  hsa04210+5613  
Calcium signaling pathway  hsa04020+5613  
Chemokine signaling pathway  hsa04062+5613  
Cholinergic synapse  hsa04725+5613  
Dopaminergic synapse  hsa04728+5613  
GABAergic synapse  hsa04727+5613  
Gap junction  hsa04540+5613  
Glutamatergic synapse  hsa04724+5613  
GnRH signaling pathway  hsa04912+5613  
Hedgehog signaling pathway  hsa04340+5613  
Insulin signaling pathway  hsa04910+5613  
Long-term potentiation  hsa04720+5613  
MAPK signaling pathway  hsa04010+5613  
Melanogenesis  hsa04916+5613  
Olfactory transduction  hsa04740+5613  
Oocyte meiosis  hsa04114+5613  
Progesterone-mediated oocyte maturation  hsa04914+5613  
Taste transduction  hsa04742+5613  
Vascular smooth muscle contraction  hsa04270+5613  
Wnt signaling pathway  hsa04310+5613  
 

Biophysicochemical properties

Kinetic parameters
KM 127 uM for ATP (in the presence of 10 mM magnesium chloride)
KM 58 uM for kemptide (in the presence of 10 mM magnesium chloride)
KM 6.7 uM for kemptide (in the presence of 1 uM Br-cAMP at 30 degrees Celsius)

Keywords

Biological process 
Angiogenesis  definition   [KW-0037]
Differentiation  definition   [KW-0221]
Molecular function 
Developmental protein  definition   [KW-0217]
Kinase  definition   [KW-0418]
Serine/threonine-protein kinase  definition   [KW-0723]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Other
GenomeRNAi: 5613
PRO: PR:P51817
Chemistry
GuidetoPHARMACOLOGY: 2175