NEK2 - Serine/threonine-protein kinase Nek2 - human protein (Function)
 
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NEK2 »  Serine/threonine-protein kinase Nek2   [ EC 2.7.11.1 ]
 
Protein also known as:  Never in mitosis A-related kinase 2 (NimA-related protein kinase 2).
Gene name:  NEK2
Entry whose protein(s) existence is based on evidence at protein level
extend overview
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GENE REF ISO

Function

 show evidences
Overview 
Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome.  
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  • UniProtKB
Isoform  Iso 2   Which is not present in the nucleolus, does not.  
14
  • UniProtKB
Isoform  Iso 1   Phosphorylates and activates NEK11 in G1/S-arrested cells.  
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GO molecular function 
ATP bindingdefinition[GO:0005524]  
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Metal ion bindingdefinition[GO:0046872]  
1
  • UniProtKB KW
Protein bindingdefinition[GO:0005515]  
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  • IntAct
Protein kinase activitydefinition[GO:0004672]  
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  • UniProtKB
Protein phosphatase bindingdefinition[GO:0019903]  
1
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Protein serine/threonine kinase activitydefinition[GO:0004674]  
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  • RefGenome
GO biological process 
Blastocyst developmentdefinition[GO:0001824] silver  
1
  • Ortholog Compara
Cell divisiondefinition[GO:0051301]  
1
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Centrosome separationdefinition[GO:0051299]  
1
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Chromosome segregationdefinition[GO:0007059]  
1
  • UniProtKB
Meiotic cell cycledefinition[GO:0051321]  
1
  • UniProtKB KW
Mitotic nuclear divisiondefinition[GO:0007067]  
1
  • PINC
Mitotic sister chromatid segregationdefinition[GO:0000070] silver  
1
  • Ortholog Compara
Mitotic spindle assemblydefinition[GO:0090307] silver  
1
  • Ortholog Compara
Negative regulation of centriole-centriole cohesiondefinition[GO:1903126]  
1
  • UniProtKB
Negative regulation of DNA bindingdefinition[GO:0043392] silver  
1
  • Ortholog Compara
Protein autophosphorylationdefinition[GO:0046777]  
2
  • UniProtKB
Protein phosphorylationdefinition[GO:0006468]  
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Regulation of attachment of spindle microtubules to kinetochoredefinition[GO:0051988]  
1
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Regulation of mitotic centrosome separationdefinition[GO:0046602]  
1
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Regulation of mitotic nuclear divisiondefinition[GO:0007088]  
1
  • PINC
Spindle assemblydefinition[GO:0051225]  
1
  • UniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.7.11.1: ATP + a protein ADP + a phosphoprotein.  
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It requires the following cofactor
 Mg(2+)  
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  • UniProtKB
It is regulated in the following manner
Isoform  Iso 1   Is inhibited by ionizing radiation in the presence of PPP1CA. Its catalytic activity is inhibited by the inhibitor CCT241950. In the presence of this inhibitor, displays an autoinhibited conformation: Tyr-70 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix.  
4
  • UniProtKB
 
More information is available from:
Pathways 
According to Reactome, this protein belongs to the following pathways:
Anchoring of the basal body to the plasma membrane  REACT_267965  
APC-Cdc20 mediated degradation of Nek2A  REACT_8017  
Loss of Nlp from mitotic centrosomes  REACT_15364  
Loss of proteins required for interphase microtubule organization from the centrosome  REACT_15451  
Recruitment of mitotic centrosome proteins and complexes  REACT_15296  
Regulation of PLK1 Activity at G2/M Transition  REACT_160315  
 

Keywords

Biological process 
Cell cycle  definition   [KW-0131]
Cell division  definition   [KW-0132]
Chromosome partition  definition   [KW-0159]
Meiosis  definition   [KW-0469]
Mitosis  definition   [KW-0498]
Molecular function 
Kinase  definition   [KW-0418]
Serine/threonine-protein kinase  definition   [KW-0723]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Other
GeneWiki: NEK2
GenomeRNAi: 4751
PRO: PR:P51955
Chemistry
GuidetoPHARMACOLOGY: 2117