NEK2 - Serine/threonine-protein kinase Nek2 - human protein (Function)
 
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NEK2 »  Serine/threonine-protein kinase Nek2   [ EC 2.7.11.1 ]
 
Protein also known as:  Never in mitosis A-related kinase 2 (NimA-related protein kinase 2).
Gene name:  NEK2
Entry whose protein(s) existence is based on evidence at protein level
extend overview
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GENE REF ISO

Function

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Overview 
Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome.  
13
  • CuratedUniProtKB
Isoform  Iso 2   Which is not present in the nucleolus, does not.  
13
  • CuratedUniProtKB
Isoform  Iso 1   Phosphorylates and activates NEK11 in G1/S-arrested cells.  
13
  • CuratedUniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
Metal ion bindingdefinition[GO:0046872]  
  • IEAUniProtKB KW
Protein bindingdefinition[GO:0005515]  
8
  • IPIIntAct
  • IPIUniProtKB
Protein kinase activitydefinition[GO:0004672]  
2
  • IDAUniProtKB
Protein phosphatase bindingdefinition[GO:0019903]  
1
  • IPIUniProtKB
Protein serine/threonine kinase activitydefinition[GO:0004674]  
1
  • TASUniProtKB
GO biological process 
Blastocyst developmentdefinition[GO:0001824] silver  
  • IEAOrtholog Compara
Centrosome separationdefinition[GO:0051299]  
1
  • IDAUniProtKB
Chromosome segregationdefinition[GO:0007059]  
1
  • IDAUniProtKB
Meiotic nuclear divisiondefinition[GO:0007126]  
  • IEAUniProtKB KW
Mitosisdefinition[GO:0007067]  
1
  • TASPINC
Mitotic sister chromatid segregationdefinition[GO:0000070] silver  
  • IEAOrtholog Compara
Negative regulation of DNA bindingdefinition[GO:0043392] silver  
  • IEAOrtholog Compara
Protein autophosphorylationdefinition[GO:0046777]  
2
  • IDAUniProtKB
Protein phosphorylationdefinition[GO:0006468]  
1
  • IDAUniProtKB
Regulation of attachment of spindle microtubules to kinetochoredefinition[GO:0051988]  
1
  • IMPUniProtKB
Regulation of mitosisdefinition[GO:0007088]  
1
  • TASPINC
Regulation of mitotic centrosome separationdefinition[GO:0046602]  
1
  • TASUniProtKB
Spindle assemblydefinition[GO:0051225]  
1
  • TASUniProtKB
Spindle assembly involved in mitosisdefinition[GO:0090307] silver  
  • IEAOrtholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.7.11.1: ATP + a protein ADP + a phosphoprotein.  
  • CuratedUniProtKB
It requires the following cofactor
Magnesium.  
  • CuratedUniProtKB
It is regulated in the following manner
Isoform  Iso 1   Is inhibited by ionizing radiation in the presence of PPP1CA. Its catalytic activity is inhibited by the inhibitor CCT241950. In the presence of this inhibitor, displays an autoinhibited conformation: Tyr-70 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix.  
3
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to Reactome, this protein belongs to the following pathways:
APC-Cdc20 mediated degradation of Nek2A  REACT_8017  
Cell Cycle  REACT_115566  
 

Keywords

Biological process 
Cell cycle  definition   [KW-0131]
Cell division  definition   [KW-0132]
Chromosome partition  definition   [KW-0159]
Meiosis  definition   [KW-0469]
Mitosis  definition   [KW-0498]
Molecular function 
Kinase  definition   [KW-0418]
Serine/threonine-protein kinase  definition   [KW-0723]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Other
GeneWiki: NEK2
GenomeRNAi: 4751
PRO: PR:P51955
Chemistry
GuidetoPHARMACOLOGY: 2117