SLC7A5 - Large neutral amino acids transporter small subunit 1 - human protein (Function)
 
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Protein
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SLC7A5 »  Large neutral amino acids transporter small subunit 1
 
Protein also known as:  y+ system cationic amino acid transporter.
Gene name:  SLC7A5
Entry whose protein(s) existence is based on evidence at protein level
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1 82 1
GENE REF ISO

Function

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Overview 
Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.  
14
  • CuratedUniProtKB
According to TCDB this is a transporter from family:
the amino acid-polyamine-organocation (apc) family  2.A.3.8.25  
GO molecular function 
Amino acid transmembrane transporter activitydefinition[GO:0015171]  
  • ISSOrtholog Curator
L-amino acid transmembrane transporter activitydefinition[GO:0015179] silver  
  • IEAOrtholog Compara
Neutral amino acid transmembrane transporter activitydefinition[GO:0015175]  
1
  • TASPINC
Peptide antigen bindingdefinition[GO:0042605]  
  • ISSOrtholog Curator
GO biological process 
Cell differentiationdefinition[GO:0030154]  
  • IEAUniProtKB KW
Cellular amino acid metabolic processdefinition[GO:0006520]  
1
  • TASPINC
Nervous system developmentdefinition[GO:0007399]  
  • IEAUniProtKB KW
Neutral amino acid transportdefinition[GO:0015804]  
  • ISSOrtholog Curator
Transportdefinition[GO:0006810]  
1
  • TASPINC
Pathways 
According to Reactome, this protein belongs to the following pathways:
Amino acid and oligopeptide SLC transporters  REACT_19419  
Hemostasis  REACT_604  
Transmembrane transport of small molecules  REACT_15518  
 
More information is available from:
Note 
The uptake of leucine, tyrosine and tryptophan is inhibited by the different iodothyronines, in particular T3. Leucine transport is also inhibited by small zwitterionic amino acids (i.e. Glycine, alanine, serine, threonine and cysteine) and by glutamine and asparginine. The uptake of T3 is almost completely blocked by coincubation with leucine, tryptophan, tyrosine, and phenylalanine, or 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH). Methionine uptake was inhibited by the L-system substrates L-leucine, BCH, L-cysteine and by the MeHg-L-cysteine complex and structurally related S-ethyl-L-cysteine. MeHg-L-cysteine uptake is inhibited by L-methionine, L-leucine, BCH and S-ethyl-L-cysteine. L-leucine uptake was inhibited by L-CNSO. Tyrosine uptake in fibroblasts was inhibited by D-methionine, and methyl-aminoisobutyric acid (MeAIB).  
  • CuratedUniProtKB
 

Biophysicochemical properties

Kinetic parameters
KM 60.4 uM for tyrosine (in T24 human bladder carcinoma cells)
KM 740 uM for L-phenylalanine
KM 55.2 uM for phenylalanine (in T24 human bladder carcinoma cells)
KM 16.4 uM for tyrosine (in human fibroblasts)
KM 96.5 uM for 3-O-methyldopa (in T24 human bladder carcinoma cells)
KM 7.9 uM for T4 (in the presence of choline chloride)
KM 153 uM for alpha-methyltyrosine (in T24 human bladder carcinoma cells)
KM 2.2 mM for L-glutamine
KM 12.5 uM for reverse triiodothyronine (rT3) (in the presence of choline chloride)
KM 99 uM for methionine
KM 35 uM for L-histidine
KM 191 uM for gabapentin (in T24 human bladder carcinoma cells)
KM 46 uM for leucine (in the presence of choline chloride)
KM 0.8 uM for T3 (in the presence of choline chloride)
KM 162 uM for thyroxine (in T24 human bladder carcinoma cells)
KM 156 uM for BCH (in T24 human bladder carcinoma cells)
KM 216 uM for alpha-methyldopa (in T24 human bladder carcinoma cells)
KM 19 uM for tryptophan (in the presence of choline chloride)
KM 98 uM for MeHg-L-cysteine
KM 138 uM for Dopa (in T24 human bladder carcinoma cells)
KM 7.9 uM for 3,3'-diiodothyronine (in the presence of choline chloride)
KM 32 uM for L-leucine
KM 10 mM for L-alanine
KM 7.3 uM for triiodothyronine (in T24 human bladder carcinoma cells)
KM 75.3 uM for melphanan (in T24 human bladder carcinoma cells)

Keywords

Biological process 
Amino-acid transport  definition   [KW-0029]
Differentiation  definition   [KW-0221]
Neurogenesis  definition   [KW-0524]
Transport  definition   [KW-0813]
Molecular function 
Developmental protein  definition   [KW-0217]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: Q01650
Other
GeneWiki: SLC7A5
GenomeRNAi: 8140
PRO: PR:Q01650