FOLH1 - Glutamate carboxypeptidase 2 - human protein (Function)
 
Home Recent activites arrow-down favorite My favorites arrow-down favorite My labels arrow-down Downloads
Back to ...  
Publication View
Protein
Gene
References

 
FOLH1 »  Glutamate carboxypeptidase 2   [ EC 3.4.17.21 ]
 
Protein also known as:  N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I).
Gene name:  FOLH1
Family name: Peptidase M28 » M28B
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 114 8
GENE REF ISO

Function

 show evidences
Overview 
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.  
  • CuratedUniProtKB
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.  
  • CuratedUniProtKB
GO molecular function 
Carboxypeptidase activitydefinition[GO:0004180] silver  
  • IEAOrtholog Compara
Dipeptidase activitydefinition[GO:0016805] silver  
  • IEAOrtholog Compara
Metal ion bindingdefinition[GO:0046872]  
  • IEAUniProtKB KW
Metallopeptidase activitydefinition[GO:0008237]  
  • IEAUniProtKB KW
Peptidase activitydefinition[GO:0008233]  
1
  • NASUniProtKB
GO biological process 
Folic acid-containing compound metabolic processdefinition[GO:0006760] silver  
  • IEAOrtholog Compara
Proteolysisdefinition[GO:0006508]  
1
  • NASUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.4.17.21: Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.  
  • CuratedUniProtKB
It requires the following cofactor
Binds 2 zinc ions per subunit. Required for NAALADase activity.  
1
  • CuratedUniProtKB
It is regulated in the following manner
The NAALADase activity is inhibited by beta-NAAG, quisqualic acid, 2-(phosphonomethyl) pentanedioic acid (PMPA) and EDTA. Activated by cobalt.  
1
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathway:
Vitamin digestion and absorption  hsa04977+2346  
Note 
PSMA is used as a diagnostic and prognostic indicator of prostate cancer, and as a possible marker for various neurological disorders such as schizophrenia, Alzheimer disease and Huntington disease.  
  • CuratedUniProtKB
 

Biophysicochemical properties

Dependence
pH Stable at pH greater than 6.5.

Keywords

Molecular function 
Carboxypeptidase  definition   [KW-0121]
Dipeptidase  definition   [KW-0224]
Hydrolase  definition   [KW-0378]
Metalloprotease  definition   [KW-0482]
Protease  definition   [KW-0645]
Technical term 
Multifunctional enzyme  definition   [KW-0511]
Reference proteome  definition   [KW-1185]
 

Further external links

Other
GeneWiki: Glutamate_carboxypeptidase_II
GenomeRNAi: 2346
PRO: PR:Q04609
Chemistry
GuidetoPHARMACOLOGY: 1606