GLO1 - Lactoylglutathione lyase - human protein (Function)
 
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GLO1 »  Lactoylglutathione lyase   [ EC 4.4.1.5 ]
 
Protein also known as:  S-D-lactoylglutathione methylglyoxal lyase.
Gene name:  GLO1
Family name: Glyoxalase I
Entry whose protein(s) existence is based on evidence at protein level
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1 54 2
GENE REF ISO

Function

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Overview 
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.  
3
  • CuratedUniProtKB
GO molecular function 
Lactoylglutathione lyase activitydefinition[GO:0004462]  
2
  • IDAUniProtKB
Zinc ion bindingdefinition[GO:0008270]  
1
  • IDAUniProtKB
GO biological process 
Carbohydrate metabolic processdefinition[GO:0005975] silver  
  • IEAOrtholog Compara
Glutathione metabolic processdefinition[GO:0006749] silver  
  • IEAOrtholog Compara
Methylglyoxal metabolic processdefinition[GO:0009438] silver  
  • IEAOrtholog Compara
Negative regulation of apoptotic processdefinition[GO:0043066]  
3
  • TASUniProtKB
Osteoclast differentiationdefinition[GO:0030316]  
  • ISSOrtholog Curator
Regulation of transcription from RNA polymerase II promoterdefinition[GO:0006357] silver  
  • IEAOrtholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 4.4.1.5: (R)-S-lactoylglutathione glutathione + methylglyoxal.  
2
  • CuratedUniProtKB
It requires the following cofactor
Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.  
2
  • CuratedUniProtKB
It is regulated in the following manner
Regulated by oxidation of Cys-139 in response to the redox state of the cell. Results in the alternative formation of cystine or glutathione-bound cysteine, the latter modification leading to reduced enzyme activity.  
1
  • CuratedUniProtKB
 
More information is available from:
Pathways 
This protein is involved in the following pathway
According to KEGG, this protein belongs to the following pathway:
Pyruvate metabolism  hsa00620+2739  
 

Biophysicochemical properties

Kinetic parameters
Reduction of GLO1 was carried out by incubation with 20 mM betamercaptoethanol prior to kinetic analysis.
KM 0.7 mM for methylglyoxal/glutathione (reduced form)
KM 1.3 mM for methylglyoxal/glutathione (native form)
Vmax 0.335 umol/min/mg enzyme with methylglyoxal/glutathione as substrate (native form)
Vmax 0.7 umol/min/mg enzyme with methylglyoxal/glutathione as substrate (reduced form)

Keywords

Molecular function 
Lyase  definition   [KW-0456]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: Q04760
Other
GeneWiki: GLO1
GeneWiki: Lactoylglutathione_lyase
GenomeRNAi: 2739
PRO: PR:Q04760