WRN - Werner syndrome ATP-dependent helicase - human protein (Function)
 
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References

 
WRN »  Werner syndrome ATP-dependent helicase   [ EC 3.6.4.12 ]
 
Protein also known as:  DNA helicase, RecQ-like type 3 (RecQ3).
Gene name:  WRN
Family name: Helicase » RecQ
Entry whose protein(s) existence is based on evidence at protein level
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1 219 1
GENE REF ISO

Function

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Overview 
Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation.  
6
  • CuratedUniProtKB
GO molecular function 
3'-5' DNA helicase activitydefinition[GO:0043138]  
1
  • IDAUniProtKB
3'-5' exonuclease activitydefinition[GO:0008408]  
4
  • IDAUniProtKB
  • IDAMGI
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
ATP-dependent 3'-5' DNA helicase activitydefinition[GO:0043140] silver  
  • IEAInterPro 2 GO
ATP-dependent DNA helicase activitydefinition[GO:0004003]  
1
  • IDAUniProtKB
ATPase activitydefinition[GO:0016887]  
2
  • IDAUniProtKB
Bubble DNA bindingdefinition[GO:0000405]  
1
  • IDAUniProtKB
DNA bindingdefinition[GO:0003677]  
1
  • IDAUniProtKB
DNA helicase activitydefinition[GO:0003678]  
2
  • IDAMGI
  • IMPUniProtKB
Exonuclease activitydefinition[GO:0004527]  
1
  • IDAMGI
Four-way junction helicase activitydefinition[GO:0009378]  
1
  • IDAUniProtKB
G-quadruplex DNA bindingdefinition[GO:0051880]  
1
  • IDAUniProtKB
Helicase activitydefinition[GO:0004386]  
1
  • IDAUniProtKB
Magnesium ion bindingdefinition[GO:0000287]  
1
  • IDAUniProtKB
Manganese ion bindingdefinition[GO:0030145]  
1
  • IDAUniProtKB
Protein bindingdefinition[GO:0005515]  
22
  • IPIUniProtKB
  • IPIIntAct
  • IPIBHF-UCL
Protein complex bindingdefinition[GO:0032403]  
1
  • IDAUniProtKB
Protein homodimerization activitydefinition[GO:0042803]  
1
  • IDAUniProtKB
Y-form DNA bindingdefinition[GO:0000403]  
1
  • IDAUniProtKB
GO biological process 
Agingdefinition[GO:0007568]  
1
  • NASUniProtKB
ATP catabolic processdefinition[GO:0006200]  
2
  • IDAGOC
  • IEAInterPro 2 GO
Base-excision repairdefinition[GO:0006284]  
1
  • IDAUniProtKB
Cell agingdefinition[GO:0007569]  
1
  • IMPUniProtKB
Cellular response to DNA damage stimulusdefinition[GO:0006974]  
1
  • IDAUniProtKB
Cellular response to gamma radiationdefinition[GO:0071480]  
1
  • IDAUniProtKB
Cellular response to starvationdefinition[GO:0009267]  
1
  • IDAMGI
DNA duplex unwindingdefinition[GO:0032508]  
5
  • IDAGOC
  • IEAInterPro 2 GO
DNA metabolic processdefinition[GO:0006259]  
1
  • IDAUniProtKB
DNA recombinationdefinition[GO:0006310] silver  
  • IEAInterPro 2 GO
DNA replicationdefinition[GO:0006260]  
1
  • IMPUniProtKB
DNA synthesis involved in DNA repairdefinition[GO:0000731]  
1
  • IDAUniProtKB
Double-strand break repairdefinition[GO:0006302]  
1
  • IMPUniProtKB
Multicellular organismal agingdefinition[GO:0010259]  
1
  • IMPUniProtKB
Nucleic acid phosphodiester bond hydrolysisdefinition[GO:0090305]  
5
  • IDAGOC
  • IEAUniProtKB KW
  • IEAInterPro 2 GO
Nucleolus to nucleoplasm transportdefinition[GO:0032066]  
1
  • IDAMGI
Positive regulation of hydrolase activitydefinition[GO:0051345]  
1
  • IDAUniProtKB
Regulation of apoptotic processdefinition[GO:0042981]  
1
  • IGIMGI
Regulation of growth ratedefinition[GO:0040009] silver  
  • IEAOrtholog Compara
Replication fork processingdefinition[GO:0031297]  
2
  • IDAUniProtKB
Replicative cell agingdefinition[GO:0001302] silver  
  • IEAOrtholog Compara
Response to oxidative stressdefinition[GO:0006979]  
1
  • IDAUniProtKB
Response to UV-Cdefinition[GO:0010225]  
1
  • IDAUniProtKB
Telomere maintenancedefinition[GO:0000723]  
1
  • IMPUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.6.4.12: ATP + H(2)O ADP + phosphate.  
1
  • CuratedUniProtKB
It requires the following cofactor
Binds 2 magnesium ions per subunit. Has high activity with manganese and zinc ions (in vitro).  
1
  • CuratedUniProtKB
 

Keywords

Biological process 
DNA damage  definition   [KW-0227]
DNA repair  definition   [KW-0234]
Molecular function 
Exonuclease  definition   [KW-0269]
Helicase  definition   [KW-0347]
Hydrolase  definition   [KW-0378]
Nuclease  definition   [KW-0540]
Technical term 
Multifunctional enzyme  definition   [KW-0511]
Reference proteome  definition   [KW-1185]
 

Further external links