WRN - Werner syndrome ATP-dependent helicase - human protein (Function)
 
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WRN »  Werner syndrome ATP-dependent helicase   [ EC 3.6.4.12 ]
 
Protein also known as:  DNA helicase, RecQ-like type 3 (RecQ3).
Gene name:  WRN
Family name: Helicase » RecQ
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation.  
7
  • UniProtKB
GO molecular function 
3'-5' DNA helicase activitydefinition[GO:0043138]  
1
  • UniProtKB
3'-5' exonuclease activitydefinition[GO:0008408]  
4
  • UniProtKB
  • MGI
ATP bindingdefinition[GO:0005524]  
1
  • UniProtKB KW
ATP-dependent 3'-5' DNA helicase activitydefinition[GO:0043140] silver  
1
  • InterPro 2 GO
ATP-dependent DNA helicase activitydefinition[GO:0004003]  
1
  • UniProtKB
ATPase activitydefinition[GO:0016887]  
2
  • UniProtKB
Bubble DNA bindingdefinition[GO:0000405]  
1
  • UniProtKB
DNA bindingdefinition[GO:0003677]  
1
  • UniProtKB
DNA helicase activitydefinition[GO:0003678]  
2
  • MGI
  • UniProtKB
Exonuclease activitydefinition[GO:0004527]  
1
  • MGI
Four-way junction helicase activitydefinition[GO:0009378]  
1
  • UniProtKB
G-quadruplex DNA bindingdefinition[GO:0051880]  
1
  • UniProtKB
Helicase activitydefinition[GO:0004386]  
1
  • UniProtKB
Magnesium ion bindingdefinition[GO:0000287]  
1
  • UniProtKB
Manganese ion bindingdefinition[GO:0030145]  
1
  • UniProtKB
Protein bindingdefinition[GO:0005515]  
23
  • IntAct
  • UniProtKB
  • BHF-UCL
Protein complex bindingdefinition[GO:0032403]  
1
  • UniProtKB
Protein homodimerization activitydefinition[GO:0042803]  
1
  • UniProtKB
Y-form DNA bindingdefinition[GO:0000403]  
1
  • UniProtKB
GO biological process 
Agingdefinition[GO:0007568]  
1
  • UniProtKB
Base-excision repairdefinition[GO:0006284]  
1
  • UniProtKB
Cell agingdefinition[GO:0007569]  
1
  • UniProtKB
Cellular response to DNA damage stimulusdefinition[GO:0006974]  
1
  • UniProtKB
Cellular response to gamma radiationdefinition[GO:0071480]  
1
  • UniProtKB
Cellular response to starvationdefinition[GO:0009267]  
1
  • MGI
DNA duplex unwindingdefinition[GO:0032508]  
6
  • GOC
  • InterPro 2 GO
DNA metabolic processdefinition[GO:0006259]  
1
  • UniProtKB
DNA recombinationdefinition[GO:0006310] silver  
1
  • InterPro 2 GO
DNA replicationdefinition[GO:0006260]  
1
  • UniProtKB
DNA synthesis involved in DNA repairdefinition[GO:0000731]  
1
  • UniProtKB
Double-strand break repairdefinition[GO:0006302]  
1
  • UniProtKB
Multicellular organismal agingdefinition[GO:0010259]  
1
  • UniProtKB
Nucleic acid phosphodiester bond hydrolysisdefinition[GO:0090305]  
7
  • GOC
  • UniProtKB KW
  • InterPro 2 GO
Nucleolus to nucleoplasm transportdefinition[GO:0032066]  
1
  • MGI
Positive regulation of hydrolase activitydefinition[GO:0051345]  
1
  • UniProtKB
Regulation of apoptotic processdefinition[GO:0042981]  
1
  • MGI
Regulation of growth ratedefinition[GO:0040009] silver  
1
  • Ortholog Compara
Replication fork processingdefinition[GO:0031297]  
2
  • UniProtKB
Replicative cell agingdefinition[GO:0001302] silver  
1
  • Ortholog Compara
Response to oxidative stressdefinition[GO:0006979]  
1
  • UniProtKB
Response to UV-Cdefinition[GO:0010225]  
1
  • UniProtKB
Telomere maintenancedefinition[GO:0000723]  
1
  • UniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.6.4.12: ATP + H(2)O ADP + phosphate.  
2
  • UniProtKB
It requires the following cofactor
 Mg(2+) : Binds 2 magnesium ions per subunit. Has high activity with manganese and zinc ions (in vitro).  
2
  • UniProtKB
 
More information is available from:
 

Keywords

Biological process 
DNA damage  definition   [KW-0227]
DNA repair  definition   [KW-0234]
Molecular function 
Exonuclease  definition   [KW-0269]
Helicase  definition   [KW-0347]
Hydrolase  definition   [KW-0378]
Nuclease  definition   [KW-0540]
Technical term 
Multifunctional enzyme  definition   [KW-0511]
Reference proteome  definition   [KW-1185]
 

Further external links