ESPL1 - Separin - human protein (Function)
 
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ESPL1 »  Separin   [ EC 3.4.22.49 ]
 
Protein also known as:  Extra spindle poles-like 1 protein.
Gene name:  ESPL1
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.  
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  • CuratedUniProtKB
GO molecular function 
Catalytic activitydefinition[GO:0003824]  
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  • NASUniProtKB
Cysteine-type peptidase activitydefinition[GO:0008234]  
1
  • NASUniProtKB
Protein bindingdefinition[GO:0005515]  
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  • IPIUniProtKB
GO biological process 
Apoptotic processdefinition[GO:0006915]  
1
  • TASUniProtKB
Cytokinesisdefinition[GO:0000910]  
1
  • NASUniProtKB
Establishment of mitotic spindle localizationdefinition[GO:0040001]  
1
  • NASUniProtKB
Homologous chromosome segregationdefinition[GO:0045143] silver  
  • IEAOrtholog Compara
Meiotic spindle organizationdefinition[GO:0000212] silver  
  • IEAOrtholog Compara
Mitotic sister chromatid segregationdefinition[GO:0000070]  
1
  • IMPUniProtKB
Negative regulation of sister chromatid cohesiondefinition[GO:0045875]  
1
  • NASUniProtKB
Positive regulation of mitotic metaphase/anaphase transitiondefinition[GO:0045842]  
1
  • NASUniProtKB
Proteolysisdefinition[GO:0006508]  
  • IEAUniProtKB KW
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.4.22.49: All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.  
  • CuratedUniProtKB
It is regulated in the following manner
Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. The association with PTTG1 is not only inhibitory, since PTTG1 is also required for activating it, the enzyme being inactive in cells in which PTTG1 is absent. PTTG1 degradation at anaphase, liberates it and triggers RAD21 cleavage. Second, phosphorylation at Ser-1126 inactivates it. The complete phosphorylation during mitosis, is removed when cells undergo anaphase. Activation of the enzyme at the metaphase-anaphase transition probably requires the removal of both securin and inhibitory phosphate.  
3
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathways:
Cell cycle  hsa04110+9700  
Oocyte meiosis  hsa04114+9700  
According to Reactome, this protein belongs to the following pathway:
Separation of Sister Chromatids  REACT_150471  
 
More information is available from:
 

Keywords

Biological process 
Chromosome partition  definition   [KW-0159]
Molecular function 
Hydrolase  definition   [KW-0378]
Protease  definition   [KW-0645]
Thiol protease  definition   [KW-0788]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: Separase
GenomeRNAi: 9700
PRO: PR:Q14674