ACOX1 - Peroxisomal acyl-coenzyme A oxidase 1 - human protein (Function)
 
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ACOX1 »  Peroxisomal acyl-coenzyme A oxidase 1   [ EC 1.3.3.6 ]  (AOX)
 
Protein also known as:  Straight-chain acyl-CoA oxidase (SCOX).
Gene name:  ACOX1
Family name: Acyl-CoA oxidase
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 34 3
GENE REF ISO

Function

 show evidences
Overview 
Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs.  
2
  • CuratedUniProtKB
Isoform  Iso 1   Shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length.  
2
  • CuratedUniProtKB
Isoform  Iso 2   Is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs.  
2
  • CuratedUniProtKB
Isoform  Iso 2   Is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.  
2
  • CuratedUniProtKB
GO molecular function 
Acyl-CoA dehydrogenase activitydefinition[GO:0003995] silver  
  • IEAInterPro 2 GO
Acyl-CoA oxidase activitydefinition[GO:0003997]  
4
  • IMPBHF-UCL
  • IGIUniProtKB
Isoforms  Iso 1 and Iso 2   FAD bindingdefinition[GO:0071949]  
1
  • IDAUniProtKB
Fatty acid bindingdefinition[GO:0005504] silver  
  • IEAOrtholog Compara
Flavin adenine dinucleotide bindingdefinition[GO:0050660] silver  
  • IEAInterPro 2 GO
Palmitoyl-CoA oxidase activitydefinition[GO:0016401]  
1
  • IDAUniProtKB
  • IEAOrtholog Compara
PDZ domain bindingdefinition[GO:0030165]  
1
  • IDAMGI
Protein N-terminus bindingdefinition[GO:0047485]  
1
  • IPIUniProtKB
Receptor bindingdefinition[GO:0005102]  
1
  • IPIUniProtKB
GO biological process 
Fatty acid beta-oxidation using acyl-CoA oxidasedefinition[GO:0033540]  
1
  • IMPBHF-UCL
  • IEAUniPathway
Fatty acid oxidationdefinition[GO:0019395]  
2
  • IGIUniProtKB
Generation of precursor metabolites and energydefinition[GO:0006091]  
1
  • IMPUniProtKB
Isoforms  Iso 1 and Iso 2   Lipid homeostasisdefinition[GO:0055088]  
2
  • IDAUniProtKB
Lipid metabolic processdefinition[GO:0006629]  
1
  • IDAUniProtKB
Isoform  Iso 2   Peroxisome fissiondefinition[GO:0016559]  
1
  • IGIUniProtKB
Isoform  Iso 1   Positive regulation of cholesterol homeostasisdefinition[GO:2000189]  
1
  • IGIUniProtKB
Prostaglandin metabolic processdefinition[GO:0006693]  
1
  • IMPUniProtKB
Spermatogenesisdefinition[GO:0007283] silver  
  • IEAOrtholog Compara
Very long-chain fatty acid metabolic processdefinition[GO:0000038]  
1
  • IMPBHF-UCL
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 1.3.3.6: Acyl-CoA + O(2) trans-2,3-dehydroacyl-CoA + H(2)O(2).  
  • CuratedUniProtKB
It requires the following cofactor
FAD.  
1
  • CuratedUniProtKB
Pathways 
This protein is involved in the following pathway
According to KEGG, this protein belongs to the following pathways:
alpha-Linolenic acid metabolism  hsa00592+51  
Biosynthesis of unsaturated fatty acids  hsa01040+51  
Fatty acid metabolism  hsa00071+51  
Metabolic pathways  hsa01100+51  
Peroxisome  hsa04146+51  
PPAR signaling pathway  hsa03320+51  
According to Reactome, this protein belongs to the following pathway:
Metabolism  REACT_111217  
 
More information is available from:
Note 
Isoform  Iso 2   Is more effective in reversal of the phenotype than isoform 1 (PubMed20195242).  
1
  • CuratedUniProtKB
Isoforms  Iso 1 and Iso 2   Can reverse the Acox1 null phenotype in mouse which is characterized by severe microvesicular hepatic steatosis, sustained activation of Ppara, spontaneous massive peroxisome proliferation and eventual development of hepatocellular carcinomas.  
  • CuratedUniProtKB
 

Biophysicochemical properties

Kinetic parameters
KM 73 uM for palmitoyl-CoA (isoform 1)
KM 90 uM for palmitoyl-CoA (isoform 2)
Dependence
pH Optimum pH is 8.5 for isoform 1 and 7.5-8.5 for isoform 2.
Temperature Optimum temperature for isoform 1 at pH 7.5 is 40 degrees Celsius with no activity at 50 degrees Celsius. Optimum temperature for isoform 2 at pH 7.5 is 47.5 degrees Celsius with 57% activity retained at 50 degrees Celsius.

Keywords

Biological process 
Fatty acid metabolism  definition   [KW-0276]
Lipid metabolism  definition   [KW-0443]
Molecular function 
Oxidoreductase  definition   [KW-0560]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: ACOX1
GenomeRNAi: 51
PRO: PR:Q15067