RGN - Regucalcin - human protein (Function)
 
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Protein
Gene
References

 
RGN »  Regucalcin  (RC)
 
Protein also known as:  Senescence marker protein 30 (SMP-30).
Gene name:  RGN
Family name: SMP-30/CGR1
Entry whose protein(s) existence is based on evidence at protein level
extend overview
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GENE REF ISO

Function

 show evidences
Overview 
Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity).  
1
  • UniProtKB
GO molecular function 
Calcium ion bindingdefinition[GO:0005509]  
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  • UniProtKB
  • Ortholog Curator
Enzyme regulator activitydefinition[GO:0030234] silver  
1
  • InterPro 2 GO
Gluconolactonase activitydefinition[GO:0004341]  
2
  • UniProtKB
Zinc ion bindingdefinition[GO:0008270]  
1
  • UniProtKB
GO biological process 
Agingdefinition[GO:0007568] silver  
1
  • Ortholog Compara
Cellular calcium ion homeostasisdefinition[GO:0006874]  
1
  • Ortholog Curator
Kidney developmentdefinition[GO:0001822] silver  
1
  • Ortholog Compara
L-ascorbic acid biosynthetic processdefinition[GO:0019853] silver  
1
  • Ortholog Compara
Liver regenerationdefinition[GO:0097421] silver  
1
  • Ortholog Compara
Negative regulation of apoptotic processdefinition[GO:0043066] silver  
1
  • Ortholog Compara
Negative regulation of bone developmentdefinition[GO:1903011] silver  
1
  • Ortholog Compara
Negative regulation of calcium-dependent ATPase activitydefinition[GO:1903611] silver  
1
  • Ortholog Compara
Negative regulation of cyclic-nucleotide phosphodiesterase activitydefinition[GO:0051344] silver  
1
  • Ortholog Compara
Negative regulation of DNA biosynthetic processdefinition[GO:2000279] silver  
1
  • Ortholog Compara
Negative regulation of DNA catabolic processdefinition[GO:1903625] silver  
1
  • Ortholog Compara
Negative regulation of epithelial cell proliferationdefinition[GO:0050680] silver  
1
  • Ortholog Compara
Negative regulation of GTPase activitydefinition[GO:0034260] silver  
1
  • Ortholog Compara
Negative regulation of leucine-tRNA ligase activitydefinition[GO:1903634] silver  
1
  • Ortholog Compara
Negative regulation of nitric oxide biosynthetic processdefinition[GO:0045019] silver  
1
  • Ortholog Compara
Negative regulation of phosphoprotein phosphatase activitydefinition[GO:0032515] silver  
1
  • Ortholog Compara
Negative regulation of protein kinase activitydefinition[GO:0006469] silver  
1
  • Ortholog Compara
Negative regulation of RNA biosynthetic processdefinition[GO:1902679] silver  
1
  • Ortholog Compara
Negative regulation of sperm motilitydefinition[GO:1901318] silver  
1
  • Ortholog Compara
Positive regulation of ATPase activitydefinition[GO:0032781]  
1
  • Ortholog Curator
Positive regulation of calcium-transporting ATPase activitydefinition[GO:1901896] silver  
1
  • Ortholog Compara
Positive regulation of dUTP diphosphatase activitydefinition[GO:1903629] silver  
1
  • Ortholog Compara
Positive regulation of fatty acid biosynthetic processdefinition[GO:0045723] silver  
1
  • Ortholog Compara
Positive regulation of glucose metabolic processdefinition[GO:0010907] silver  
1
  • Ortholog Compara
Positive regulation of GTPase activitydefinition[GO:0043547] silver  
1
  • Ortholog Compara
Positive regulation of phosphatase activitydefinition[GO:0010922] silver  
1
  • Ortholog Compara
Positive regulation of proteolysis involved in cellular protein catabolic processdefinition[GO:1903052] silver  
1
  • Ortholog Compara
Positive regulation of superoxide dismutase activitydefinition[GO:1901671] silver  
1
  • Ortholog Compara
Positive regulation of triglyceride biosynthetic processdefinition[GO:0010867] silver  
1
  • Ortholog Compara
Regulation of calcium-mediated signalingdefinition[GO:0050848]  
1
  • Ortholog Curator
Spermatogenesisdefinition[GO:0007283] silver  
1
  • Ortholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.1.1.17: D-glucono-1,5-lactone + H(2)O D-gluconate.  
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  • UniProtKB
It requires the following cofactor
 Zn(2+) : Binds 1 divalent metal cation per subunit. Most active with Zn(2+) and Mn(2+) ions. The physiological cofactor is most likely Ca(2+) or Mg(2+).  
3
  • UniProtKB
Pathways 
According to KEGG, this protein belongs to the following pathways:
Ascorbate and aldarate metabolism  hsa00053+9104  
Metabolic pathways  hsa01100+9104  
Pentose phosphate pathway  hsa00030+9104  
Caution 
Gluconolactonase catalyzes a key step in ascorbic acid (vitamin C) biosynthesis, but primates lack the last enzyme in the pathway and are unable to synthesize vitamin C.  
1
  • UniProtKB
 

Biophysicochemical properties

Kinetic parameters
KM 0.6 mM for gluconolactone (with Mn(2+) as cofactor)
KM 2.7 mM for gluconolactone (with Zn(2+) as cofactor)
KM 3.7 mM for gluconolactone (with Ca(2+) as cofactor)
KM 1.3 mM for gluconolactone (with Mg(2+) as cofactor)

Keywords

Molecular function 
Hydrolase  definition   [KW-0378]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: RGN_(gene)
GenomeRNAi: 9104
PRO: PR:Q15493