PKN1 - Serine/threonine-protein kinase N1 - human protein (Function)
 
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PKN1 »  Serine/threonine-protein kinase N1   [ EC 2.7.11.13 ]
 
Protein also known as:  Protein-kinase C-related kinase 1.
Gene name:  PKN1
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 102 3
GENE REF ISO

Function

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Overview 
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.  
11
  • UniProtKB
GO molecular function 
Androgen receptor bindingdefinition[GO:0050681]  
2
  • UniProtKB
ATP bindingdefinition[GO:0005524]  
1
  • UniProtKB KW
Chromatin bindingdefinition[GO:0003682]  
1
  • UniProtKB
GTP-Rho bindingdefinition[GO:0017049]  
2
  • UniProtKB
Histone bindingdefinition[GO:0042393]  
1
  • UniProtKB
Histone deacetylase bindingdefinition[GO:0042826]  
1
  • UniProtKB
Histone kinase activity (H3-T11 specific)definition[GO:0035402]  
1
  • UniProtKB
Ligand-dependent nuclear receptor transcription coactivator activitydefinition[GO:0030374]  
2
  • UniProtKB
Protein bindingdefinition[GO:0005515]  
5
  • IntAct
  • UniProtKB
Protein kinase activitydefinition[GO:0004672]  
2
  • PINC
Protein kinase C activitydefinition[GO:0004697]  
1
  • EC 2 GO
Protein kinase C bindingdefinition[GO:0005080]  
1
  • UniProtKB
Protein serine/threonine kinase activitydefinition[GO:0004674]  
1
  • UniProtKB
Rac GTPase bindingdefinition[GO:0048365]  
1
  • UniProtKB
GO biological process 
Activation of JUN kinase activitydefinition[GO:0007257]  
1
  • PINC
Epithelial cell migrationdefinition[GO:0010631]  
1
  • UniProtKB
Histone H3-T11 phosphorylationdefinition[GO:0035407]  
1
  • UniProtKB
Hyperosmotic responsedefinition[GO:0006972] silver  
1
  • Ortholog Compara
Protein phosphorylationdefinition[GO:0006468]  
1
  • UniProtKB
Regulation of cell motilitydefinition[GO:2000145]  
1
  • UniProtKB
Regulation of transcription from RNA polymerase II promoterdefinition[GO:0006357]  
2
  • UniProtKB
Signal transductiondefinition[GO:0007165]  
1
  • PINC
Transcription, DNA-templateddefinition[GO:0006351]  
1
  • UniProtKB KW
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.7.11.13: ATP + a protein ADP + a phosphoprotein.  
2
  • UniProtKB
It is regulated in the following manner
Kinase activity is activated upon binding to Rho proteins (RHOA, RHOB and RAC1). Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by caspase-3 (CASP3) cleavage during apoptosis. Two specific sites, Thr-774 (activation loop of the kinase domain) and Ser-916 (turn motif), need to be phosphorylated for its full activation.  
3
  • UniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathway:
Salmonella infection  hsa05132+5585  
 

Biophysicochemical properties

Kinetic parameters
KM 20.6 uM for HDAC5

Keywords

Biological process 
Transcription  definition   [KW-0804]
Transcription regulation  definition   [KW-0805]
Molecular function 
Chromatin regulator  definition   [KW-0156]
Kinase  definition   [KW-0418]
Serine/threonine-protein kinase  definition   [KW-0723]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Other
GeneWiki: Protein_kinase_N1
GenomeRNAi: 5585
PRO: PR:Q16512
Chemistry
GuidetoPHARMACOLOGY: 1520