PKN1 - Serine/threonine-protein kinase N1 - human protein (Function)
 
Home Recent activites arrow-down favorite My favorites arrow-down favorite My labels arrow-down Downloads
Back to ...  
Publication View
Protein
Gene
References

 
PKN1 »  Serine/threonine-protein kinase N1   [ EC 2.7.11.13 ]
 
Protein also known as:  Serine-threonine protein kinase N.
Gene name:  PKN1
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 95 3
GENE REF ISO

Function

 show evidences
Overview 
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.  
10
  • CuratedUniProtKB
GO molecular function 
Androgen receptor bindingdefinition[GO:0050681]  
2
  • IDAUniProtKB
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
Chromatin bindingdefinition[GO:0003682]  
1
  • IDAUniProtKB
GTP-Rho bindingdefinition[GO:0017049]  
2
  • IDAUniProtKB
Histone bindingdefinition[GO:0042393]  
1
  • IDAUniProtKB
Histone deacetylase bindingdefinition[GO:0042826]  
1
  • IDAUniProtKB
Histone kinase activity (H3-T11 specific)definition[GO:0035402]  
1
  • IDAUniProtKB
Ligand-dependent nuclear receptor transcription coactivator activitydefinition[GO:0030374]  
2
  • IMPUniProtKB
Protein bindingdefinition[GO:0005515]  
4
  • IPIUniProtKB
  • IPIIntAct
Protein kinase activitydefinition[GO:0004672]  
2
  • TASPINC
Protein kinase C activitydefinition[GO:0004697]  
  • IEAEC 2 GO
Protein kinase C bindingdefinition[GO:0005080]  
1
  • IPIUniProtKB
Protein serine/threonine kinase activitydefinition[GO:0004674]  
1
  • IDAUniProtKB
Rac GTPase bindingdefinition[GO:0048365]  
1
  • IDAUniProtKB
GO biological process 
Activation of JUN kinase activitydefinition[GO:0007257]  
1
  • TASPINC
Epithelial cell migrationdefinition[GO:0010631]  
1
  • IMPUniProtKB
Histone H3-T11 phosphorylationdefinition[GO:0035407]  
1
  • IDAUniProtKB
Hyperosmotic responsedefinition[GO:0006972] silver  
  • IEAOrtholog Compara
Protein phosphorylationdefinition[GO:0006468]  
1
  • IDAUniProtKB
Regulation of cell motilitydefinition[GO:2000145]  
1
  • IMPUniProtKB
Regulation of transcription from RNA polymerase II promoterdefinition[GO:0006357]  
2
  • IMPUniProtKB
Signal transductiondefinition[GO:0007165]  
1
  • TASPINC
Transcription, DNA-templateddefinition[GO:0006351]  
  • IEAUniProtKB KW
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.7.11.13: ATP + a protein ADP + a phosphoprotein.  
1
  • CuratedUniProtKB
It is regulated in the following manner
Kinase activity is activated upon binding to Rho proteins (RHOA, RHOB and RAC1). Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by caspase-3 (CASP3) cleavage during apoptosis. Two specific sites, Thr-774 (activation loop of the kinase domain) and Ser-916 (turn motif), need to be phosphorylated for its full activation.  
2
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathway:
Salmonella infection  hsa05132+5585  
 

Biophysicochemical properties

Kinetic parameters
KM 20.6 uM for HDAC5

Keywords

Biological process 
Transcription  definition   [KW-0804]
Transcription regulation  definition   [KW-0805]
Molecular function 
Chromatin regulator  definition   [KW-0156]
Kinase  definition   [KW-0418]
Serine/threonine-protein kinase  definition   [KW-0723]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Other
GeneWiki: Protein_kinase_N1
GenomeRNAi: 5585
PRO: PR:Q16512
Chemistry
GuidetoPHARMACOLOGY: 1520