CYP1B1 - Cytochrome P450 1B1 - human protein (Function)
 
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CYP1B1 »  Cytochrome P450 1B1   [ EC 1.14.14.1 ]
 
Protein also known as:  CYPIB1.
Gene name:  CYP1B1
Family name: Cytochrome P450
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 239 1
GENE REF ISO

Function

 show evidences
Overview 
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta-estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.  
5
  • UniProtKB
GO molecular function 
Aromatase activitydefinition[GO:0070330]  
1
  • EC 2 GO
Heme bindingdefinition[GO:0020037]  
2
  • UniProtKB
Iron ion bindingdefinition[GO:0005506] silver  
1
  • InterPro 2 GO
Monooxygenase activitydefinition[GO:0004497]  
3
  • UniProtKB
Oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygendefinition[GO:0016712]  
1
  • MGI
Oxygen bindingdefinition[GO:0019825]  
1
  • UniProtKB
GO biological process 
Angiogenesisdefinition[GO:0001525] silver  
1
  • Ortholog Compara
Arachidonic acid metabolic processdefinition[GO:0019369]  
1
  • UniProtKB
Blood vessel morphogenesisdefinition[GO:0048514]  
1
  • Ortholog Curator
Cell adhesiondefinition[GO:0007155]  
1
  • Ortholog Curator
Cellular aromatic compound metabolic processdefinition[GO:0006725] silver  
1
  • Ortholog Compara
Cellular response to hydrogen peroxidedefinition[GO:0070301]  
1
  • Ortholog Curator
Cellular response to organic cyclic compounddefinition[GO:0071407]  
1
  • MGI
Collagen fibril organizationdefinition[GO:0030199]  
1
  • Ortholog Curator
Endothelial cell migrationdefinition[GO:0043542]  
1
  • Ortholog Curator
Endothelial cell-cell adhesiondefinition[GO:0071603] silver  
1
  • Ortholog Compara
Estrogen metabolic processdefinition[GO:0008210]  
1
  • UniProtKB
Intrinsic apoptotic signaling pathway in response to oxidative stressdefinition[GO:0008631]  
1
  • Ortholog Curator
Membrane lipid catabolic processdefinition[GO:0046466]  
1
  • Ortholog Curator
Negative regulation of cell adhesion mediated by integrindefinition[GO:0033629]  
1
  • Ortholog Curator
Negative regulation of cell migrationdefinition[GO:0030336]  
1
  • Ortholog Curator
Negative regulation of cell proliferationdefinition[GO:0008285]  
1
  • Ortholog Curator
Negative regulation of NF-kappaB transcription factor activitydefinition[GO:0032088]  
1
  • Ortholog Curator
Nitric oxide biosynthetic processdefinition[GO:0006809]  
1
  • Ortholog Curator
Oxidation-reduction processdefinition[GO:0055114]  
1
  • UniProtKB
Positive regulation of angiogenesisdefinition[GO:0045766]  
1
  • Ortholog Curator
Positive regulation of apoptotic processdefinition[GO:0043065]  
1
  • Ortholog Curator
Positive regulation of JAK-STAT cascadedefinition[GO:0046427]  
1
  • Ortholog Curator
Positive regulation of vascular endothelial growth factor productiondefinition[GO:0010575]  
1
  • Ortholog Curator
Regulation of reactive oxygen species metabolic processdefinition[GO:2000377]  
1
  • Ortholog Curator
Response to toxic substancedefinition[GO:0009636] silver  
1
  • Ortholog Compara
Retinal blood vessel morphogenesisdefinition[GO:0061304]  
1
  • Ortholog Curator
Retinal metabolic processdefinition[GO:0042574]  
1
  • UniProtKB
Retinol metabolic processdefinition[GO:0042572]  
1
  • UniProtKB
Steroid metabolic processdefinition[GO:0008202]  
1
  • UniProtKB
Toxin metabolic processdefinition[GO:0009404] silver  
1
  • Ortholog Compara
Trabecular meshwork developmentdefinition[GO:0002930]  
1
  • Ortholog Curator
Visual perceptiondefinition[GO:0007601]  
1
  • UniProtKB
Xenobiotic metabolic processdefinition[GO:0006805]  
1
  • UniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 1.14.14.1: RH + reduced flavoprotein + O(2) ROH + oxidized flavoprotein + H(2)O.  
3
  • UniProtKB
It requires the following cofactor
 heme  
2
  • UniProtKB
It is regulated in the following manner
Enzyme activity is increased by liposomes containing anionic phospholipids, phosphatidic acid and cardiolipin. Inhibited by naringenin with an IC(50) of 5 uM.  
3
  • UniProtKB
Pathways 
According to KEGG, this protein belongs to the following pathways:
Metabolism of xenobiotics by cytochrome P450  hsa00980+1545  
Steroid hormone biosynthesis  hsa00140+1545  
Tryptophan metabolism  hsa00380+1545  
According to Reactome, this protein belongs to the following pathways:
Defective CYP1B1 causes Glaucoma  REACT_268087  
Endogenous sterols  REACT_13812  
Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)  REACT_150134  
Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)  REACT_150417  
 
More information is available from:
 

Biophysicochemical properties

Kinetic parameters
kcat is 0.15 min(-1) for retinol, 0.77 min(-1) for retinal, 2.86 min(-1) for 7,12-dimethyltetraphene, 0.48 min(-1) for arachidonic acid.
KM 212.8 uM for 7,12-dimethyltetraphene
KM 29.8 uM for arachidonic acid
KM 24.0 uM for progesterone
KM 8.5 uM for retinal
KM 6.0 uM for 17-beta-estradiol
KM 18.5 uM for retinol
KM 17.0 uM for testosterone
Vmax 36.16 nmol/min/mg enzyme for testosterone 6-beta-hydroxylation
Vmax 14.95 nmol/min/mg enzyme for 17-beta-estradiol 4-hydroxylation
Vmax 6.9 nmol/min/mg enzyme for 17-beta-estradiol 2-hydroxylation
Vmax 37.80 nmol/min/mg enzyme for progesterone 16-alpha-hydroxylation
Vmax 9.86 nmol/min/mg enzyme for progesterone 6-beta-hydroxylation

Keywords

Molecular function 
Monooxygenase  definition   [KW-0503]
Oxidoreductase  definition   [KW-0560]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: Q16678
Other
GeneWiki: CYP1B1
GenomeRNAi: 1545
PRO: PR:Q16678