CYP1B1 - Cytochrome P450 1B1 - human protein (Function)
 
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CYP1B1 »  Cytochrome P450 1B1   [ EC 1.14.14.1 ]
 
Protein also known as:  CYPIB1.
Gene name:  CYP1B1
Family name: Cytochrome P450
Entry whose protein(s) existence is based on evidence at protein level
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1 220 1
GENE REF ISO

Function

 show evidences
Overview 
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta-estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.  
4
  • CuratedUniProtKB
GO molecular function 
Aromatase activitydefinition[GO:0070330]  
  • IEAEC 2 GO
Heme bindingdefinition[GO:0020037]  
2
  • IDAUniProtKB
Iron ion bindingdefinition[GO:0005506] silver  
  • IEAInterPro 2 GO
Monooxygenase activitydefinition[GO:0004497]  
3
  • IDAUniProtKB
Oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygendefinition[GO:0016712]  
1
  • IDAMGI
Oxygen bindingdefinition[GO:0019825]  
1
  • TASUniProtKB
GO biological process 
Angiogenesisdefinition[GO:0001525] silver  
  • IEAOrtholog Compara
Arachidonic acid metabolic processdefinition[GO:0019369]  
1
  • IDAUniProtKB
Blood vessel morphogenesisdefinition[GO:0048514]  
  • ISSOrtholog Curator
Cell adhesiondefinition[GO:0007155]  
  • ISSOrtholog Curator
Cellular aromatic compound metabolic processdefinition[GO:0006725] silver  
  • IEAOrtholog Compara
Cellular response to hydrogen peroxidedefinition[GO:0070301]  
  • ISSOrtholog Curator
Cellular response to organic cyclic compounddefinition[GO:0071407]  
1
  • IDAMGI
Collagen fibril organizationdefinition[GO:0030199]  
  • ISSOrtholog Curator
Endothelial cell migrationdefinition[GO:0043542]  
  • ISSOrtholog Curator
Endothelial cell-cell adhesiondefinition[GO:0071603] silver  
  • IEAOrtholog Compara
Estrogen metabolic processdefinition[GO:0008210]  
1
  • IDAUniProtKB
Intrinsic apoptotic signaling pathway in response to oxidative stressdefinition[GO:0008631]  
  • ISSOrtholog Curator
Membrane lipid catabolic processdefinition[GO:0046466]  
  • ISSOrtholog Curator
Negative regulation of cell adhesion mediated by integrindefinition[GO:0033629]  
  • ISSOrtholog Curator
Negative regulation of cell migrationdefinition[GO:0030336]  
  • ISSOrtholog Curator
Negative regulation of cell proliferationdefinition[GO:0008285]  
  • ISSOrtholog Curator
Negative regulation of NF-kappaB transcription factor activitydefinition[GO:0032088]  
  • ISSOrtholog Curator
Nitric oxide biosynthetic processdefinition[GO:0006809]  
  • ISSOrtholog Curator
Oxidation-reduction processdefinition[GO:0055114]  
1
  • TASUniProtKB
Positive regulation of angiogenesisdefinition[GO:0045766]  
  • ISSOrtholog Curator
Positive regulation of apoptotic processdefinition[GO:0043065]  
  • ISSOrtholog Curator
Positive regulation of gene expression involved in extracellular matrix organizationdefinition[GO:1901313]  
  • ISSOrtholog Curator
Positive regulation of JAK-STAT cascadedefinition[GO:0046427]  
  • ISSOrtholog Curator
Positive regulation vascular endothelial growth factor productiondefinition[GO:0010575]  
  • ISSOrtholog Curator
Regulation of reactive oxygen species metabolic processdefinition[GO:2000377]  
  • ISSOrtholog Curator
Response to toxic substancedefinition[GO:0009636] silver  
  • IEAOrtholog Compara
Retinal blood vessel morphogenesisdefinition[GO:0061304]  
  • ISSOrtholog Curator
Retinal metabolic processdefinition[GO:0042574]  
1
  • IDAUniProtKB
Retinol metabolic processdefinition[GO:0042572]  
1
  • IDAUniProtKB
Steroid metabolic processdefinition[GO:0008202]  
1
  • IDAUniProtKB
Toxin metabolic processdefinition[GO:0009404] silver  
  • IEAOrtholog Compara
Trabecular meshwork developmentdefinition[GO:0002930]  
  • ISSOrtholog Curator
Visual perceptiondefinition[GO:0007601]  
1
  • TASUniProtKB
Xenobiotic metabolic processdefinition[GO:0006805]  
1
  • IDAUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 1.14.14.1: RH + reduced flavoprotein + O(2) ROH + oxidized flavoprotein + H(2)O.  
2
  • CuratedUniProtKB
It requires the following cofactor
Heme group.  
1
  • CuratedUniProtKB
It is regulated in the following manner
Enzyme activity is increased by liposomes containing anionic phospholipids, phosphatidic acid and cardiolipin. Inhibited by naringenin with an IC(50) of 5 uM.  
2
  • CuratedUniProtKB
Pathways 
According to KEGG, this protein belongs to the following pathways:
Metabolism of xenobiotics by cytochrome P450  hsa00980+1545  
Steroid hormone biosynthesis  hsa00140+1545  
Tryptophan metabolism  hsa00380+1545  
According to Reactome, this protein belongs to the following pathways:
Endogenous sterols  REACT_13812  
Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)  REACT_150134  
Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)  REACT_150417  
 
More information is available from:
 

Biophysicochemical properties

Kinetic parameters
kcat is 0.15 min(-1) for retinol, 0.77 min(-1) for retinal, 2.86 min(-1) for 7,12-dimethyltetraphene, 0.48 min(-1) for arachidonic acid.
KM 212.8 uM for 7,12-dimethyltetraphene
KM 29.8 uM for arachidonic acid
KM 24.0 uM for progesterone
KM 8.5 uM for retinal
KM 6.0 uM for 17-beta-estradiol
KM 18.5 uM for retinol
KM 17.0 uM for testosterone
Vmax 36.16 nmol/min/mg enzyme for testosterone 6-beta-hydroxylation
Vmax 14.95 nmol/min/mg enzyme for 17-beta-estradiol 4-hydroxylation
Vmax 6.9 nmol/min/mg enzyme for 17-beta-estradiol 2-hydroxylation
Vmax 37.80 nmol/min/mg enzyme for progesterone 16-alpha-hydroxylation
Vmax 9.86 nmol/min/mg enzyme for progesterone 6-beta-hydroxylation

Keywords

Molecular function 
Monooxygenase  definition   [KW-0503]
Oxidoreductase  definition   [KW-0560]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: Q16678
Other
GeneWiki: CYP1B1
GenomeRNAi: 1545
PRO: PR:Q16678