GEN1 - Flap endonuclease GEN homolog 1 - human protein (Function)
 
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Protein
Gene
References

 
GEN1 »  Flap endonuclease GEN homolog 1
 
Gene name:  GEN1
Family name: XPG/RAD2 endonuclease » GEN
Entry whose protein(s) existence is based on evidence at protein level
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1 30 1
GENE REF ISO

Function

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Overview 
Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation.  
1
  • CuratedUniProtKB
GO molecular function 
Crossover junction endodeoxyribonuclease activitydefinition[GO:0008821]  
1
  • IMPUniProtKB
DNA bindingdefinition[GO:0003677] silver  
  • IEAInterPro 2 GO
Metal ion bindingdefinition[GO:0046872]  
  • IEAUniProtKB KW
GO biological process 
DNA catabolic process, endonucleolyticdefinition[GO:0000737]  
1
  • IMPGOC
Double-strand break repair via homologous recombinationdefinition[GO:0000724]  
1
  • IMPUniProtKB
Positive regulation of mitotic cell cycle spindle assembly checkpointdefinition[GO:0090267]  
1
  • IMPUniProtKB
Regulation of centrosome duplicationdefinition[GO:0010824]  
2
  • IMPUniProtKB
Resolution of mitotic recombination intermediatesdefinition[GO:0071140]  
1
  • IMPUniProtKB
Resolution of recombination intermediatesdefinition[GO:0071139]  
1
  • IMPUniProtKB
Enzymatic activity 
It requires the following cofactor
Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding (By similarity).  
  • CuratedUniProtKB
 

Keywords

Biological process 
DNA damage  definition   [KW-0227]
DNA repair  definition   [KW-0234]
Molecular function 
Endonuclease  definition   [KW-0255]
Hydrolase  definition   [KW-0378]
Nuclease  definition   [KW-0540]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GenomeRNAi: 348654
PRO: PR:Q17RS7