We identified and cloned a novel gene encoding a partner protein, CIP150, of connexin 43 (Cx43). CIP150 associates with Cx43 through its carboxyl terminal domain. Conversely, a region consisting of 16 amino acids in the juxtamembrane region (amino acids 227-242) in the carboxyl terminal tail of Cx43 was identified to be responsible for the association. A variant of Cx43 lacking this region was expressed only in a nonphosphorylated form and appeared to lose the capacity to localize to the region of cell-cell contact and dye transfer activity. When the expression of CIP150 was suppressed using small interfering RNA, the localization to the plasma membrane as well as dye transfer activity of Cx43 was significantly reduced. These results suggest that the newly identified domain is essential for the proper phosphorylation and localization of Cx43, and CIP150 is a novel partner protein targeting this domain.
A reference proteome is a set of protein sequences derived from a complete proteome which constitutes a defined standard for a particular user community. Reference proteomes are manually defined according to a number of criteria. They cover the proteomes of well- studied model organisms and other proteomes of interest for biomedical and biotechnological research. Reference proteomes have been selected to provide broad coverage of the tree of life, and constitute a representative cross-section of the taxonomic diversity to be found within UniProtKB.
My favorites 
My labels 
Login
