ACER2 - Alkaline ceramidase 2 - human protein (Function)
 
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ACER2 »  Alkaline ceramidase 2   [ EC 3.5.1.23 ]  (AlkCDase 2)
 
Protein also known as:  N-acylsphingosine amidohydrolase 3-like.
Gene name:  ACER2
Family name: Alkaline ceramidase
Entry whose protein(s) existence is based on evidence at protein level
extend overview
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GENE REF ISO

Function

 show evidences
Overview 
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)-ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)-ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate.  
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  • CuratedUniProtKB
GO molecular function 
Ceramidase activitydefinition[GO:0017040]  
2
  • IDAUniProtKB
Dihydroceramidase activitydefinition[GO:0071633]  
1
  • IDABHF-UCL
GO biological process 
Activation of cysteine-type endopeptidase activity involved in apoptotic processdefinition[GO:0006919]  
1
  • IMPBHF-UCL
Cellular response to drugdefinition[GO:0035690]  
1
  • IEPBHF-UCL
Ceramide metabolic processdefinition[GO:0006672] silver  
  • IEAInterPro 2 GO
Negative regulation of cell adhesion mediated by integrindefinition[GO:0033629]  
1
  • IDAUniProtKB
Negative regulation of cell-matrix adhesiondefinition[GO:0001953]  
1
  • IDAUniProtKB
Negative regulation of protein glycosylation in Golgidefinition[GO:0090285]  
1
  • IMPUniProtKB
Positive regulation of cell deathdefinition[GO:0010942]  
1
  • IMPBHF-UCL
Positive regulation of cell proliferationdefinition[GO:0008284]  
1
  • IDAUniProtKB
Response to retinoic aciddefinition[GO:0032526]  
1
  • IDAUniProtKB
Sphingosine biosynthetic processdefinition[GO:0046512]  
3
  • IDABHF-UCL
  • IDAUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.5.1.23: N-acylsphingosine + H(2)O a carboxylate + sphingosine.  
  • CuratedUniProtKB
It is regulated in the following manner
Specifically activated by lumenal, but not cytosolic Ca(2+). Inhibited by Zn(2+) or Cu(2+). Mg(2+) or Mn(2+) have no effect on ceramidase activity.  
1
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathways:
Metabolic pathways  hsa01100+340485  
Sphingolipid metabolism  hsa00600+340485  
According to Reactome, this protein belongs to the following pathway:
Metabolism  REACT_111217  
 

Biophysicochemical properties

Kinetic parameters
KM 81 uM for D-erythro-C(24:1)-ceramide (at 37 degrees Celsius and pH 9.0)
Vmax 27 pmol/min/mg enzyme with D-erythro-C(24:1)-ceramide as substrate
Dependence
pH Optimum pH is 7.5-9.0.

Keywords

Biological process 
Lipid metabolism  definition   [KW-0443]
Molecular function 
Hydrolase  definition   [KW-0378]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: ACER2
GenomeRNAi: 340485
PRO: PR:Q5QJU3