ATAT1 - Alpha-tubulin N-acetyltransferase 1 - human protein (Function)
 
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ATAT1 »  Alpha-tubulin N-acetyltransferase 1   [ EC 2.3.1.108 ]  (TAT)
 
Protein also known as:  Acetyltransferase mec-17 homolog.
Gene name:  ATAT1
Family name: UPF0635
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 17 7
GENE REF ISO

Function

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Overview 
Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Required for normal sperm flagellar function. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.  
3
  • CuratedUniProtKB
GO molecular function 
Tubulin N-acetyltransferase activitydefinition[GO:0019799]  
1
  • IMPUniProtKB
GO biological process 
Alpha-tubulin acetylationdefinition[GO:0071929]  
  • IEAUniProtKB Annot
Neuron developmentdefinition[GO:0048666]  
  • IEAUniProtKB Annot
Regulation of microtubule cytoskeleton organizationdefinition[GO:0070507]  
  • IEAUniProtKB Annot
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.3.1.108: Acetyl-CoA + [alpha-tubulin]-L-lysine CoA + [alpha-tubulin]-N(6)-acetyl-L-lysine.  
1
  • CuratedUniProtKB
 

Biophysicochemical properties

Kinetic parameters
kcat is 2.2 h(-1) for the acetylation of polymerized tubulin (microtubules), 0.35 h(-1) for the acetylation of free tubulin, and 1.47 h(-1)with acetyl-CoA as substrate.
KM 2.2 uM for acetyl-CoA
KM 1.6 uM for polymerized tubulin (microtubules)
KM 2.0 uM for free alpha-tubulin

Keywords

Molecular function 
Acyltransferase  definition   [KW-0012]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GenomeRNAi: 79969
PRO: PR:Q5SQI0