LPCAT3 - Lysophospholipid acyltransferase 5 - human protein (Function)
 
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LPCAT3 »  Lysophospholipid acyltransferase 5   [ EC 2.3.1.- ]  (LPLAT 5)
 
Protein also known as:  Membrane-bound O-acyltransferase domain-containing protein 5 (O-acyltransferase domain-containing protein 5).
Gene name:  LPCAT3
Entry whose protein(s) existence is based on evidence at protein level
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1 28 2
GENE REF ISO

Function

 show evidences
Overview 
Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (LPCAT activity). Catalyzes also the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity). Has also weak lysophosphatidylethanolamine acyltransferase activity (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.  
2
  • CuratedUniProtKB
GO molecular function 
1-acylglycerophosphocholine O-acyltransferase activitydefinition[GO:0047184]  
1
  • IMPMGI
GO biological process 
Regulation of plasma lipoprotein particle levelsdefinition[GO:0097006] silver  
  • IEAOrtholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
Acyl-CoA + 1-acyl-sn-glycero-3-phosphatidylserine CoA + 1,2-diacyl-sn-glycero-3-phosphatidylserine.  
  • CuratedUniProtKB
EC 2.3.1.23: Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine CoA + 1,2-diacyl-sn-glycero-3-phosphocholine.  
  • CuratedUniProtKB
It is regulated in the following manner
Activity is inhibited by thimerosal.  
  • CuratedUniProtKB
Pathways 
This protein is involved in the following pathway
According to KEGG, this protein belongs to the following pathway:
Glycerophospholipid metabolism  hsa00564+10162  
According to Reactome, this protein belongs to the following pathways:
Acyl chain remodelling of PC  REACT_120829  
Acyl chain remodelling of PE  REACT_121369  
Acyl chain remodelling of PS  REACT_121384  
 

Biophysicochemical properties

Kinetic parameters
KM 201.4 uM for linoleoyl-CoA
KM 71.56 uM for arachidonoyl-CoA
KM 72.68 uM for oleoyl-CoA
KM 41.29 uM for palmitoyl-CoA
KM 36.65 uM for stearoyl-CoA
KM 72.19 uM for 1-palmitoyl-lysophosphatidylcholine
Vmax 18148 nmol/min/mg enzyme with linoleoyl-CoA and 1-palmitoyl-lysophosphatidylcholine as substrates
Vmax 4698 nmol/min/mg enzyme with oleoyl-CoA and 1-palmitoyl-lysophosphatidylcholine as substrates
Vmax 996 nmol/min/mg enzyme with stearoyl-CoA and 1-palmitoyl-lysophosphatidylcholine as substrates
Vmax 1782 nmol/min/mg enzyme with palmitoyl-CoA and 1-palmitoyl-lysophosphatidylcholine as substrates
Vmax 6247 nmol/min/mg enzyme with arachidonoyl-CoA and 1-palmitoyl-lysophosphatidylcholine as substrates

Keywords

Biological process 
Lipid biosynthesis  definition   [KW-0444]
Lipid metabolism  definition   [KW-0443]
Phospholipid biosynthesis  definition   [KW-0594]
Phospholipid metabolism  definition   [KW-1208]
Molecular function 
Acyltransferase  definition   [KW-0012]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: MBOAT5
GenomeRNAi: 10162
PRO: PR:Q6P1A2