Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins (By similarity).
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
J. Cell Biol. 154, 447-458 (2001)[PubMed:11470831]
The epithelial-mesenchymal interactions required for kidney organogenesis are disrupted in mice lacking the integrin alpha8beta1. None of this integrin's known ligands, however, appears to account for this phenotype. To identify a more relevant ligand, a soluble integrin alpha8beta1 heterodimer fused to alkaline phosphatase (AP) has been used to probe blots and cDNA libraries. In newborn mouse kidney extracts, alpha8beta1-AP detects a novel ligand of 70-90 kD. This protein, named nephronectin, is an extracellular matrix protein with five EGF-like repeats, a mucin region containing a RGD sequence, and a COOH-terminal MAM domain. Integrin alpha8beta1 and several additional RGD-binding integrins bind nephronectin. Nephronectin mRNA is expressed in the ureteric bud epithelium, whereas alpha8beta1 is expressed in the metanephric mesenchyme. Nephronectin is localized in the extracellular matrix in the same distribution as the ligand detected by alpha8beta1-AP and forms a complex with alpha8beta1 in vivo. Thus, these results strongly suggest that nephronectin is a relevant ligand mediating alpha8beta1 function in the kidney. Nephronectin is expressed at numerous sites outside the kidney, so it may also have wider roles in development. The approaches used here should be generally useful for characterizing the interactions of novel extracellular matrix proteins identified through genomic sequencing projects.
The process in which the branching structure of the ureteric bud is generated and organized. The ureteric bud is an epithelial tube that grows out from the metanephric duct. The bud elongates and branches to give rise to the ureter and kidney collecting tubules.
Evidence
1:
Inferred from Sequence or Structural SimilarityBHF-UCL
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
The process in which relatively unspecialized cells, e.g. embryonic or regenerative cells, acquire specialized structural and/or functional features that characterize the cells, tissues, or organs of the mature organism or some other relatively stable phase of the organism's life history. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state.
The attachment of one cell to another cell via an integrin, a heterodimeric adhesion receptor formed by the non-covalent association of particular alpha and beta subunits.
Evidence
1:
Inferred from Sequence or Structural SimilarityBHF-UCL
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a tumor necrosis factor stimulus.
Evidence
1:
Inferred from Sequence or Structural SimilarityBHF-UCL
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of an extracellular matrix.
Evidence
1:
Inferred from Sequence or Structural SimilarityBHF-UCL
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
Any process that increases the frequency, rate or extent of cell-substrate adhesion. Cell-substrate adhesion is the attachment of a cell to the underlying substrate via adhesion molecules.
Evidence
1:
Inferred from Sequence or Structural SimilarityBHF-UCL
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
Int. J. Mol. Med. 15, 719-724 (2005)[PubMed:15754038]
We describe the identification and characterization of a human epidermal growth factor (EGF)-like gene, nephronectin (NPNT), which encodes predicted proteins most homologous to the mouse nephronectin (Npnt). Human NPNT is located at the chromosomal position 4q25. Two transcript variants, NPNTa and NPNTb, are identified. Sequence comparison indicated that the human NPNT proteins share 88% amino acid identity with their mouse homologs. The in silico expression analysis showed the NPNT expression in a number of embryonic and adult tissues. NPNT was expressed in fetal ear, eye, heart, lung and kidney. In adults, the NPNT expression was widely found in lung, kidney, brain, uterus, placenta, thyroid gland and blood vessels. This gene is therefore implicated in embryonic development as well as maintenance of various adult tissues.
Protein involved in differentiation, the developmental process of a multicellular organism by which cells become specialized for particular functions. Differentiation requires selective expression of the genome; the fully differentiated state may be preceded by a stage in which the cell is already programmed for differentiation but is not yet expressing the characteristic phenotype determination. Also used for fungal conidiation proteins, and for some bacteria that present specialization of function in cell types, such as Caulobacter crescentus.
Protein involved in development, the process whereby a multicellular organism develops from its early immature forms, e.g., zygote, larva, embryo, into an adult.
A reference proteome is a set of protein sequences derived from a complete proteome which constitutes a defined standard for a particular user community. Reference proteomes are manually defined according to a number of criteria. They cover the proteomes of well- studied model organisms and other proteomes of interest for biomedical and biotechnological research. Reference proteomes have been selected to provide broad coverage of the tree of life, and constitute a representative cross-section of the taxonomic diversity to be found within UniProtKB.
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