ATG4D - Cysteine protease ATG4D - human protein (Function)
 
Home Recent activites arrow-down favorite My favorites arrow-down favorite My labels arrow-down Downloads
Back to ...  
Publication View
Protein
Gene
References

 
ATG4D »  Cysteine protease ATG4D   [ EC 3.4.22.- ]
 
Protein also known as:  Autophagy-related cysteine endopeptidase 4. Cleaved into:  Cysteine protease ATG4D, mitochondrial.
Gene name:  ATG4D
Family name: Peptidase C54
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 13 1
GENE REF ISO

Function

 show evidences
Overview 
Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.  
3
  • CuratedUniProtKB
Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability.  
3
  • CuratedUniProtKB
GO molecular function 
Cysteine-type endopeptidase activitydefinition[GO:0004197]  
1
  • ISSUniProtKB
GO biological process 
Apoptotic processdefinition[GO:0006915]  
  • IEAUniProtKB KW
Autophagydefinition[GO:0006914]  
  • IEAUniProtKB KW
Protein transportdefinition[GO:0015031]  
  • IEAUniProtKB KW
Proteolysisdefinition[GO:0006508]  
  • IEAUniProtKB KW
Enzymatic activity 
It is regulated in the following manner
Inhibited by N-ethylmaleimide.  
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathway:
Regulation of autophagy  hsa04140+84971  
 

Biophysicochemical properties

Kinetic parameters
KM 7.2 uM for GABARAPL2
KM 13.1 uM for MAP1LC3B

Keywords

Biological process 
Apoptosis  definition   [KW-0053]
Autophagy  definition   [KW-0072]
Protein transport  definition   [KW-0653]
Transport  definition   [KW-0813]
Ubl conjugation pathway  definition   [KW-0833]
Molecular function 
Hydrolase  definition   [KW-0378]
Protease  definition   [KW-0645]
Thiol protease  definition   [KW-0788]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GenomeRNAi: 84971
PRO: PR:Q86TL0