ACOT1 - Acyl-coenzyme A thioesterase 1 - human protein (Function)
 
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Protein
Gene
References

 
ACOT1 »  Acyl-coenzyme A thioesterase 1
 
Gene name:  ACOT1
Entry whose protein(s) existence is based on evidence at protein level
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1 7 1
GENE REF ISO

Function

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Overview 
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 (By similarity).  
  • CuratedUniProtKB
GO molecular function 
Acyl-CoA hydrolase activitydefinition[GO:0047617]  
1
  • IDAHGNC
Palmitoyl-CoA hydrolase activitydefinition[GO:0016290]  
  • IEAEC 2 GO
GO biological process 
Acyl-CoA metabolic processdefinition[GO:0006637]  
1
  • IDAHGNC
Long-chain fatty acid metabolic processdefinition[GO:0001676]  
1
  • IDAHGNC
Very long-chain fatty acid metabolic processdefinition[GO:0000038]  
1
  • IDAHGNC
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.1.2.2: Palmitoyl-CoA + H(2)O CoA + palmitate.  
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathways:
Biosynthesis of unsaturated fatty acids  hsa01040+641371  
Fatty acid elongation  hsa00062+641371  
 
More information is available from:
 

Biophysicochemical properties

Kinetic parameters
KM 2 uM for C20-acyl-CoA
KM 2.8 uM for C14-acyl-CoA
KM 35.8 uM for C10-acyl-CoA
KM 4.1 uM for C18:1-acyl-CoA
KM 3.6 uM for C16-acyl-CoA
KM 2.1 uM for C18:1-trans-acyl-CoA
KM 2.4 uM for C16:1-acyl-CoA
KM 2.4 uM for C18-acyl-CoA
KM 3.6 uM for C12-acyl-CoA
Vmax 577 nmol/min/mg enzyme toward C16:1-acyl-CoA
Vmax 520 nmol/min/mg enzyme toward C20-acyl-CoA
Vmax 691 nmol/min/mg enzyme toward C16-acyl-CoA
Vmax 700 nmol/min/mg enzyme toward C12-acyl-CoA
Vmax 912 nmol/min/mg enzyme toward C14-acyl-CoA
Vmax 309 nmol/min/mg enzyme toward C18:1-trans-acyl-CoA
Vmax 258 nmol/min/mg enzyme toward C18:1-acyl-CoA
Vmax 224 nmol/min/mg enzyme toward C10-acyl-CoA
Vmax 597 nmol/min/mg enzyme toward C18-acyl-CoA

Keywords

Molecular function 
Hydrolase  definition   [KW-0378]
Serine esterase  definition   [KW-0719]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: Q86TX2
Other
GenomeRNAi: 641371
PRO: PR:Q86TX2