APLF - Aprataxin and PNK-like factor - human protein (Function)
 
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APLF »  Aprataxin and PNK-like factor   [ EC 4.2.99.18 ]
 
Protein also known as:  PNK and APTX-like FHA domain-containing protein.
Gene name:  APLF
Family name: APLF
Entry whose protein(s) existence is based on evidence at protein level
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1 27 1
GENE REF ISO

Function

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Overview 
Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.  
2
  • CuratedUniProtKB
GO molecular function 
3'-5' exonuclease activitydefinition[GO:0008408]  
1
  • IDAUniProtKB
DNA-(apurinic or apyrimidinic site) lyase activitydefinition[GO:0003906]  
1
  • IDAUniProtKB
Endodeoxyribonuclease activitydefinition[GO:0004520]  
1
  • IDAUniProtKB
Metal ion bindingdefinition[GO:0046872]  
  • IEAUniProtKB KW
Nucleotide bindingdefinition[GO:0000166]  
1
  • IDAUniProtKB
Protein bindingdefinition[GO:0005515]  
4
  • IPIIntAct
  • IPIUniProtKB
GO biological process 
Cellular response to DNA damage stimulusdefinition[GO:0006974]  
1
  • IDAUniProtKB
DNA catabolic processdefinition[GO:0006308]  
1
  • IDAGOC
DNA catabolic process, endonucleolyticdefinition[GO:0000737]  
1
  • IDAGOC
Double-strand break repairdefinition[GO:0006302]  
1
  • IMPUniProtKB
Nucleic acid phosphodiester bond hydrolysisdefinition[GO:0090305]  
1
  • IDAGOC
Positive regulation of DNA ligationdefinition[GO:0051106]  
1
  • IGIMGI
Single strand break repairdefinition[GO:0000012]  
1
  • IMPUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 4.2.99.18: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.  
1
  • CuratedUniProtKB
 

Keywords

Biological process 
DNA damage  definition   [KW-0227]
DNA repair  definition   [KW-0234]
Molecular function 
Lyase  definition   [KW-0456]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GenomeRNAi: 200558
PRO: PR:Q8IW19