ACOT4 - Acyl-coenzyme A thioesterase 4 - human protein (Function)
 
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ACOT4 »  Acyl-coenzyme A thioesterase 4   [ EC 3.1.2.2 ]  (Acyl-CoA thioesterase 4)
 
Protein also known as:  Peroxisomal acyl coenzyme A thioester hydrolase Ib.
Gene name:  ACOT4
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 11 1
GENE REF ISO

Function

 show evidences
Overview 
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (By similarity). Succinyl-CoA thioesterase that also hydrolyzes long chain saturated and unsaturated monocarboxylic acyl-CoAs.  
  • CuratedUniProtKB
GO molecular function 
Acyl-CoA hydrolase activitydefinition[GO:0047617]  
2
  • IDAHGNC
  • IDAUniProtKB
Palmitoyl-CoA hydrolase activitydefinition[GO:0016290]  
  • IEAEC 2 GO
Receptor bindingdefinition[GO:0005102]  
1
  • IPIUniProtKB
Succinyl-CoA hydrolase activitydefinition[GO:0004778]  
1
  • IDAUniProtKB
GO biological process 
Acyl-CoA metabolic processdefinition[GO:0006637]  
1
  • IDAHGNC
Not  Butyrate metabolic processdefinition[GO:0019605]  
1
  • IDAHGNC
Dicarboxylic acid catabolic processdefinition[GO:0043649]  
1
  • IDAUniProtKB
Dicarboxylic acid metabolic processdefinition[GO:0043648]  
1
  • IDAHGNC
Long-chain fatty acid metabolic processdefinition[GO:0001676]  
1
  • IDAHGNC
Saturated monocarboxylic acid metabolic processdefinition[GO:0032788]  
1
  • IDAHGNC
Short-chain fatty acid metabolic processdefinition[GO:0046459]  
1
  • IDAHGNC
Succinyl-CoA metabolic processdefinition[GO:0006104]  
1
  • IDAHGNC
Unsaturated monocarboxylic acid metabolic processdefinition[GO:0032789]  
1
  • IDAHGNC
Very long-chain fatty acid metabolic processdefinition[GO:0000038]  
1
  • IDAHGNC
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.1.2.2: Palmitoyl-CoA + H(2)O CoA + palmitate.  
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathways:
Biosynthesis of unsaturated fatty acids  hsa01040+122970  
Fatty acid elongation  hsa00062+122970  
 
More information is available from:
Note 
Human ACOT4 may have acquired the combined activities of mouse ACOT3, ACOT4, and ACOT5.  
  • CuratedUniProtKB
 

Biophysicochemical properties

Kinetic parameters
KM 3.4 uM for C14-acyl-CoA
KM 14 uM for succinyl-CoA
KM 147 uM for glutaryl-CoA
Vmax 581 nmol/min/mg enzyme toward succinyl-CoA
Vmax 132 nmol/min/mg enzyme toward glutaryl-CoA
Vmax 137 nmol/min/mg enzyme toward C14-acyl-CoA

Keywords

Molecular function 
Hydrolase  definition   [KW-0378]
Serine esterase  definition   [KW-0719]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: Q8N9L9
Other
GeneWiki: ACOT4
GenomeRNAi: 122970
PRO: PR:Q8N9L9