ATG4A - Cysteine protease ATG4A - human protein (Function)
 
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ATG4A »  Cysteine protease ATG4A   [ EC 3.4.22.- ]
 
Protein also known as:  Autophagy-related cysteine endopeptidase 2.
Gene name:  ATG4A
Family name: Peptidase C54
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.  
6
  • CuratedUniProtKB
GO molecular function 
Cysteine-type peptidase activitydefinition[GO:0008234]  
2
  • IDAUniProtKB
  • IDAMGI
GO biological process 
Autophagydefinition[GO:0006914]  
  • IEAUniProtKB KW
Protein transportdefinition[GO:0015031]  
  • IEAUniProtKB KW
Proteolysisdefinition[GO:0006508]  
2
  • IDAUniProtKB
Enzymatic activity 
It is regulated in the following manner
Inhibited by N-ethylmaleimide. Redox-regulated during autophagy since reducing conditions activate ATG4A whereas an oxidizing environment such as the presence of H(2)O(2) inhibits its activity.  
2
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathway:
Regulation of autophagy  hsa04140+115201  
 

Biophysicochemical properties

Kinetic parameters
KM 33.9 uM for MAP1LC3B
KM 20.8 uM for GABARAP
KM 36.7 uM for GABARAPL1
KM 15.7 uM for GABARAPL2

Keywords

Biological process 
Autophagy  definition   [KW-0072]
Protein transport  definition   [KW-0653]
Transport  definition   [KW-0813]
Ubl conjugation pathway  definition   [KW-0833]
Molecular function 
Hydrolase  definition   [KW-0378]
Protease  definition   [KW-0645]
Thiol protease  definition   [KW-0788]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: ATG4A
GenomeRNAi: 115201
PRO: PR:Q8WYN0