NT5C3B - 7-methylguanosine phosphate-specific 5'-nucleotidase - human protein (Function)
 
Home Recent activites arrow-down favorite My favorites arrow-down favorite My labels arrow-down Downloads
Protein
Gene
References

 
NT5C3B »  7-methylguanosine phosphate-specific 5'-nucleotidase   [ EC 3.1.3.91 ]  (7-methylguanosine nucleotidase)
 
Protein also known as:  Cytosolic 5'-nucleotidase III-like protein [EC 3.1.3.5] (cN-III-like protein).
Gene name:  NT5C3B
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 9 2
GENE REF ISO

Function

 show evidences
Overview 
Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate (PubMed23223233, PubMed24603684). The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids (PubMed23223233). Also has weak activity for CMP (PubMed23223233, PubMed24603684). UMP and purine nucleotides are poor substrates (PubMed23223233).  
3
  • UniProtKB
GO molecular function 
5'-nucleotidase activitydefinition[GO:0008253]  
1
  • EC 2 GO
Magnesium ion bindingdefinition[GO:0000287] silver  
1
  • InterPro 2 GO
Nucleotide bindingdefinition[GO:0000166]  
1
  • UniProtKB KW
Protein bindingdefinition[GO:0005515]  
1
  • IntAct
GO biological process 
Dephosphorylationdefinition[GO:0016311]  
2
  • InterPro 2 GO
  • EC 2 GO
Nucleotide metabolic processdefinition[GO:0009117]  
1
  • UniProtKB KW
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
N(7)-methyl-GMP + H(2)O N(7)-methyl-guanosine + phosphate.  
2
  • UniProtKB
CMP + H(2)O cytidine + phosphate.  
2
  • UniProtKB
EC 3.1.3.5: A 5'-ribonucleotide + H(2)O a ribonucleoside + phosphate.  
2
  • UniProtKB
Pathways 
According to Reactome, this protein belongs to the following pathway:
mRNA decay by 3' to 5' exoribonuclease  R-HSA-429958  
 

Biophysicochemical properties

Kinetic parameters
kcat is 0.24 sec(-1) with m(7)GMP. kcat is 7 sec(-1) with CMP. kcat is 0.07 sec(-1) with GMP. kcat is 0.04 sec(-1) with AMP. kcat is 6.2 sec(-1) with UMP.
KM 7.8 uM for m(7)GMP (at 37 degrees Celsius)
KM 439 uM for UMP (at 37 degrees Celsius)
KM 355 uM for GMP (at 37 degrees Celsius)
KM 8 uM for m(7)GMP (at 37 degrees Celsius)
KM 79 uM for CMP (at 37 degrees Celsius)
KM 456 uM for AMP (at 37 degrees Celsius)
Vmax 0.07 umol/min/mg enzyme with AMP as substrate
Vmax 0.13 umol/min/mg enzyme with GMP as substrate
Vmax 0.41 umol/min/mg enzyme with m(7)GMP as substrate
Vmax 10.7 umol/min/mg enzyme with UMP as substrate
Vmax 12 umol/min/mg enzyme with CMP as substrate

Keywords

Biological process 
Nucleotide metabolism  definition   [KW-0546]
Molecular function 
Hydrolase  definition   [KW-0378]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: Q969T7
Other
GenomeRNAi: 115024
PRO: PR:Q969T7