PYCR2 - Pyrroline-5-carboxylate reductase 2 - human protein (Function)
 
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Protein
Gene
References

 
PYCR2 »  Pyrroline-5-carboxylate reductase 2   [ EC 1.5.1.2 ]  (P5CR 2)
 
Gene name:  PYCR2
Entry whose protein(s) existence is based on evidence at protein level
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1 29 1
GENE REF ISO

Function

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Overview 
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH.  
2
  • CuratedUniProtKB
GO molecular function 
Pyrroline-5-carboxylate reductase activitydefinition[GO:0004735]  
  • IEAEC 2 GO
GO biological process 
L-proline biosynthetic processdefinition[GO:0055129]  
  • IEAUniPathway
Oxidation-reduction processdefinition[GO:0055114]  
  • IEAUniProtKB KW
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 1.5.1.2: L-proline + NAD(P)(+) 1-pyrroline-5-carboxylate + NAD(P)H.  
2
  • CuratedUniProtKB
It is regulated in the following manner
Subject to competitive inhibition by NADP. Not inhibited by proline.  
2
  • CuratedUniProtKB
Pathways 
This protein is involved in the following pathway
According to KEGG, this protein belongs to the following pathways:
Arginine and proline metabolism  hsa00330+29920  
Metabolic pathways  hsa01100+29920  
 

Biophysicochemical properties

Kinetic parameters
KM 0.04 mM for NADPH
KM 0.23 mM for pyrroline-5-carboxylate (in the presence of NADPH)
KM 1.49 mM for pyrroline-5-carboxylate (in the presence of NADH)
KM 0.64 mM for NADH
Vmax 28.5 umol/min/ug enzyme (in the presence of NADH)
Vmax 3.7 umol/min/ug enzyme (in the presence of NADPH)

Keywords

Biological process 
Amino-acid biosynthesis  definition   [KW-0028]
Proline biosynthesis  definition   [KW-0641]
Molecular function 
Oxidoreductase  definition   [KW-0560]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: Q96C36
Other
GenomeRNAi: 29920
PRO: PR:Q96C36