MASTL - Serine/threonine-protein kinase greatwall - human protein (Function)
 
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MASTL »  Serine/threonine-protein kinase greatwall   [ EC 2.7.11.1 ]  (GW)
 
Protein also known as:  Microtubule-associated serine/threonine-protein kinase-like (MAST-L).
Gene name:  MASTL
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.  
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  • UniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
1
  • UniProtKB KW
Kinase activitydefinition[GO:0016301]  
1
  • UniProtKB
Protein phosphatase 2A bindingdefinition[GO:0051721]  
1
  • Ortholog Curator
Protein serine/threonine kinase activitydefinition[GO:0004674]  
1
  • UniProtKB
GO biological process 
Cell divisiondefinition[GO:0051301]  
1
  • UniProtKB KW
Cellular response to DNA damage stimulusdefinition[GO:0006974]  
1
  • Ortholog Curator
G2/M transition of mitotic cell cycledefinition[GO:0000086]  
2
  • UniProtKB
Mitotic nuclear divisiondefinition[GO:0007067]  
1
  • UniProtKB
Negative regulation of protein phosphatase type 2A activitydefinition[GO:0034048]  
1
  • UniProtKB
Protein phosphorylationdefinition[GO:0006468] silver  
1
  • InterPro 2 GO
Regulation of cell cycledefinition[GO:0051726]  
1
  • UniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.7.11.1: ATP + a protein ADP + a phosphoprotein.  
2
  • UniProtKB
Pathways 
According to Reactome, this protein belongs to the following pathway:
MASTL Facilitates Mitotic Progression  REACT_150182  
Note 
Reduced levels of MASTL by RNAi causes mitotic abnormalities that consist of delay in G(2) phase and slow chromosome condensation. Cells that enter and progress through mitosis often fail to completely separate their sister chromatids in anaphase leading to the formation of 4N G(1) cells subsequent to failure of cytokinesis (PubMed:20818157 and PubMed:20538976).  
1
  • UniProtKB
 

Keywords

Biological process 
Cell cycle  definition   [KW-0131]
Cell division  definition   [KW-0132]
Mitosis  definition   [KW-0498]
Molecular function 
Kinase  definition   [KW-0418]
Serine/threonine-protein kinase  definition   [KW-0723]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: MASTL
GenomeRNAi: 84930
PRO: PR:Q96GX5