CNDP2 - Cytosolic non-specific dipeptidase - human protein (Function)
 
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CNDP2 »  Cytosolic non-specific dipeptidase   [ EC 3.4.13.18 ]
 
Protein also known as:  Glutamate carboxypeptidase-like protein 1.
Gene name:  CNDP2
Family name: Peptidase M20A
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 33 2
GENE REF ISO

Function

 show evidences
Overview 
Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly.  
2
  • CuratedUniProtKB
Isoform  Iso 2   May be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells.  
2
  • CuratedUniProtKB
GO molecular function 
Dipeptidase activitydefinition[GO:0016805] silver  
  • IEAInterPro 2 GO
Metal ion bindingdefinition[GO:0046872]  
  • IEAUniProtKB KW
Metallopeptidase activitydefinition[GO:0008237]  
  • IEAUniProtKB KW
Tripeptidase activitydefinition[GO:0034701] silver  
  • IEAInterPro 2 GO
GO biological process 
Proteolysisdefinition[GO:0006508] silver  
  • IEAOrtholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.4.13.18: Hydrolysis of dipeptides, preferentially hydrophobic dipeptides including prolyl amino acids.  
1
  • CuratedUniProtKB
It requires the following cofactor
Binds 2 manganese ions per subunit (By similarity).  
  • CuratedUniProtKB
It is regulated in the following manner
Inhibited by p-hydroxymercurybenzoate. The inhibitory concentration 50% (IC(50)) is 13 uM. Inhibited by bestatin. The inhibitory concentration 50% (IC(50)) is 7 nM at pH 9.5.  
1
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathways:
Arginine and proline metabolism  hsa00330+55748  
beta-Alanine metabolism  hsa00410+55748  
Histidine metabolism  hsa00340+55748  
Metabolic pathways  hsa01100+55748  
According to Reactome, this protein belongs to the following pathways:
Glutathione synthesis and recycling  REACT_6960  
Sulfur amino acid metabolism  REACT_115639  
 

Biophysicochemical properties

Kinetic parameters
KM 0.6 mM for Cys-Gly (at pH 8.0 and in the presence of 50 uM manganese ions)
KM 15 mM for carnosine (at pH 9.5 and in the presence of 0.1 mM manganese ions)
KM 1.04 mM for Ser-Gln (at pH 7.5 and in the presence of 0.1 mM manganese ions)

Keywords

Molecular function 
Carboxypeptidase  definition   [KW-0121]
Hydrolase  definition   [KW-0378]
Metalloprotease  definition   [KW-0482]
Protease  definition   [KW-0645]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

Enzyme and pathway databases
SABIO-RK: Q96KP4
Other
GenomeRNAi: 55748
PRO: PR:Q96KP4