USP28 - Ubiquitin carboxyl-terminal hydrolase 28 - human protein (Function)
 
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USP28 »  Ubiquitin carboxyl-terminal hydrolase 28   [ EC 3.4.19.12 ]
 
Protein also known as:  Ubiquitin-specific-processing protease 28.
Gene name:  USP28
Family name: Peptidase C19 » USP28
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus.  
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  • UniProtKB
GO molecular function 
Cysteine-type endopeptidase activitydefinition[GO:0004197]  
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  • RefGenome
Protein bindingdefinition[GO:0005515]  
3
  • UniProtKB
Ubiquitin-specific protease activitydefinition[GO:0004843]  
2
  • UniProtKB
GO biological process 
Cell proliferationdefinition[GO:0008283]  
1
  • UniProtKB
Cellular response to DNA damage stimulusdefinition[GO:0006974]  
1
  • UniProtKB
Cellular response to UVdefinition[GO:0034644]  
1
  • UniProtKB
DNA damage checkpointdefinition[GO:0000077]  
1
  • UniProtKB
DNA repairdefinition[GO:0006281]  
1
  • UniProtKB KW
Intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediatordefinition[GO:0042771]  
1
  • UniProtKB
Proteasome-mediated ubiquitin-dependent protein catabolic processdefinition[GO:0043161]  
1
  • RefGenome
Protein deubiquitinationdefinition[GO:0016579]  
3
  • UniProtKB
Regulation of proteasomal protein catabolic processdefinition[GO:0061136]  
1
  • RefGenome
Response to ionizing radiationdefinition[GO:0010212]  
1
  • UniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.4.19.12: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).  
1
  • UniProtKB
 
More information is available from:
 

Keywords

Biological process 
DNA damage  definition   [KW-0227]
DNA repair  definition   [KW-0234]
Ubl conjugation pathway  definition   [KW-0833]
Molecular function 
Hydrolase  definition   [KW-0378]
Protease  definition   [KW-0645]
Thiol protease  definition   [KW-0788]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GenomeRNAi: 57646
PRO: PR:Q96RU2