PNKP - Bifunctional polynucleotide phosphatase/kinase - human protein (Function)
 
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PNKP »  Bifunctional polynucleotide phosphatase/kinase
 
Protein also known as:  Polynucleotide kinase-3'-phosphatase.
Gene name:  PNKP
Family name: In the N-terminal section DNA 3' phosphatase
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 47 2
GENE REF ISO

Function

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Overview 
Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.  
1
  • CuratedUniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
1
  • NASUniProtKB
ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activitydefinition[GO:0046404]  
1
  • IDAUniProtKB
Damaged DNA bindingdefinition[GO:0003684]  
1
  • NASUniProtKB
Double-stranded DNA bindingdefinition[GO:0003690]  
1
  • TASUniProtKB
Endonuclease activitydefinition[GO:0004519]  
1
  • NASUniProtKB
Nucleotide kinase activitydefinition[GO:0019201]  
2
  • IDAUniProtKB
Polynucleotide 3'-phosphatase activitydefinition[GO:0046403]  
2
  • IDAUniProtKB
Protein bindingdefinition[GO:0005515]  
2
  • IPIIntAct
Purine nucleotide bindingdefinition[GO:0017076]  
1
  • NASUniProtKB
GO biological process 
Dephosphorylationdefinition[GO:0016311]  
1
  • IDAUniProtKB
DNA damage response, detection of DNA damagedefinition[GO:0042769]  
2
  • IDAUniProtKB
DNA repairdefinition[GO:0006281]  
1
  • IGIUniProtKB
DNA-dependent DNA replicationdefinition[GO:0006261]  
1
  • NASUniProtKB
Nucleic acid phosphodiester bond hydrolysisdefinition[GO:0090305]  
1
  • NASGOC
Nucleotide phosphorylationdefinition[GO:0046939]  
1
  • IDAUniProtKB
Nucleotide-excision repair, DNA damage removaldefinition[GO:0000718]  
1
  • NASUniProtKB
Polynucleotide 3' dephosphorylationdefinition[GO:0098506]  
2
  • IDAGOC
  • IEAEC 2 GO
Response to oxidative stressdefinition[GO:0006979]  
1
  • IDAUniProtKB
Response to radiationdefinition[GO:0009314]  
2
  • NASUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC 3.1.3.32: A 3'-phosphopolynucleotide + H(2)O a polynucleotide + phosphate.  
  • CuratedUniProtKB
EC 2.7.1.78: ATP + 5'-dephospho-DNA ADP + 5'-phospho-DNA.  
  • CuratedUniProtKB
 

Keywords

Biological process 
DNA damage  definition   [KW-0227]
DNA repair  definition   [KW-0234]
Molecular function 
Hydrolase  definition   [KW-0378]
Kinase  definition   [KW-0418]
Transferase  definition   [KW-0808]
Technical term 
Multifunctional enzyme  definition   [KW-0511]
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: PNKP
GenomeRNAi: 11284
PRO: PR:Q96T60