PTGES2 - Prostaglandin E synthase 2 - human protein (Function)
 
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PTGES2 »  Prostaglandin E synthase 2
 
Protein also known as:  Membrane-associated prostaglandin E synthase-2 (mPGE synthase-2). Cleaved into:  Prostaglandin E synthase 2 truncated form.
Gene name:  PTGES2
Family name: GST
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2).  
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  • UniProtKB
GO molecular function 
DNA bindingdefinition[GO:0003677] silver  
1
  • Ortholog Compara
Electron carrier activitydefinition[GO:0009055] silver  
1
  • InterPro 2 GO
Glutathione bindingdefinition[GO:0043295]  
1
  • Ortholog Curator
Heme bindingdefinition[GO:0020037]  
1
  • Ortholog Curator
Lyase activitydefinition[GO:0016829]  
1
  • Ortholog Curator
Prostaglandin-E synthase activitydefinition[GO:0050220]  
1
  • EC 2 GO
Protein bindingdefinition[GO:0005515]  
1
  • IntAct
Protein disulfide oxidoreductase activitydefinition[GO:0015035] silver  
1
  • InterPro 2 GO
GO biological process 
Cell redox homeostasisdefinition[GO:0045454] silver  
1
  • InterPro 2 GO
Oxidation-reduction processdefinition[GO:0055114]  
1
  • InterPro 2 GO
Positive regulation of transcription, DNA-templateddefinition[GO:0045893] silver  
1
  • Ortholog Compara
Prostaglandin biosynthetic processdefinition[GO:0001516]  
1
  • UniPathway
Secretiondefinition[GO:0046903] silver  
1
  • Ortholog Compara
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 5.3.99.3: (5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate (5Z,13E)-(15S)-11-alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate.  
3
  • UniProtKB
It is regulated in the following manner
Isomerase activity is increased by sulfhydril compounds. Dithiothreitol (DTT) is most effective, followed by dihydrolipoic acid, glutathione (GSH) and 2-mercaptoethanol.  
2
  • UniProtKB
 
More information is available from:
Pathways 
This protein is involved in the following pathway
Lipid metabolism; prostaglandin biosynthesis  
1
  • UniProtKB
According to KEGG, this protein belongs to the following pathways:
Arachidonic acid metabolism  hsa00590+80142  
Metabolic pathways  hsa01100+80142  
 
More information is available from:
Caution 
Heme and GST are not required for prostaglandin synthase activity, but the protein copurifies with heme and GST when DTT is omitted during the purification procedure. The GSH-heme complex-bound enzyme (mPGES-2h) has been proposed to act as a lyase and catalyze the degradation of prostaglandin E2 H2 (PGH2) to 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid (HHT) and malondialdehyde (MDA), but this activity may well be spurious. According to PubMed18198127, boiling the enzyme leads to loss of prostaglandin synthase activity, but does not eliminate the lyase activity. Besides, free heme can catalyze the formation of 12L-hydroxy-5,8,10-heptadecatrienoic acid (HHT).  
2
  • UniProtKB
 

Keywords

Biological process 
Fatty acid biosynthesis  definition   [KW-0275]
Fatty acid metabolism  definition   [KW-0276]
Lipid biosynthesis  definition   [KW-0444]
Lipid metabolism  definition   [KW-0443]
Prostaglandin biosynthesis  definition   [KW-0643]
Prostaglandin metabolism  definition   [KW-0644]
Molecular function 
Isomerase  definition   [KW-0413]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: PTGES2
GenomeRNAi: 80142
PRO: PR:Q9H7Z7