XPNPEP1 - Xaa-Pro aminopeptidase 1 - human protein (Function)
 
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XPNPEP1 »  Xaa-Pro aminopeptidase 1   [ EC 3.4.11.9 ]
 
Protein also known as:  X-prolyl aminopeptidase 1, soluble.
Gene name:  XPNPEP1
Family name: Peptidase M24B
Entry whose protein(s) existence is based on evidence at protein level
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1 33 3
GENE REF ISO

Function

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Overview 
Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro.  
  • CuratedUniProtKB
GO molecular function 
Aminopeptidase activitydefinition[GO:0004177]  
1
  • IDAUniProtKB
Manganese ion bindingdefinition[GO:0030145]  
1
  • IDAUniProtKB
Metalloaminopeptidase activitydefinition[GO:0070006]  
1
  • IDAUniProtKB
Protein homodimerization activitydefinition[GO:0042803]  
1
  • IPIUniProtKB
GO biological process 
Bradykinin catabolic processdefinition[GO:0010815]  
1
  • IDAUniProtKB
Proteolysisdefinition[GO:0006508]  
1
  • IDAUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.4.11.9: Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.  
2
  • CuratedUniProtKB
It requires the following cofactor
Binds 2 manganese ions per subunit.  
2
  • CuratedUniProtKB
It is regulated in the following manner
Inhibited by apstatin and the metal ion chelators EDTA and 1,10-phenanthroline. Partially inhibited by dithiothreitol. Not inhibited by enalaprilat or amastatin.  
1
  • CuratedUniProtKB
 
More information is available from:
 

Biophysicochemical properties

Kinetic parameters
KM 100.6 uM for bradykinin
KM 308 uM for the tripeptide Arg-Pro-Pro
Dependence
pH Optimum pH is 8.2.

Keywords

Molecular function 
Aminopeptidase  definition   [KW-0031]
Hydrolase  definition   [KW-0378]
Metalloprotease  definition   [KW-0482]
Protease  definition   [KW-0645]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: XPNPEP1
GenomeRNAi: 7511
PRO: PR:Q9NQW7