DROSHA - Ribonuclease 3 - human protein (Function)
 
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Protein
Gene
References

 
DROSHA »  Ribonuclease 3   [ EC 3.1.26.3 ]
 
Protein also known as:  Ribonuclease III (RNase III).
Gene name:  DROSHA
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 95 4
GENE REF ISO

Function

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Overview 
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.  
9
  • CuratedUniProtKB
GO molecular function 
Lipopolysaccharide bindingdefinition[GO:0001530]  
1
  • IDAUniProtKB
Metal ion bindingdefinition[GO:0046872]  
  • IEAUniProtKB KW
Poly(A) RNA bindingdefinition[GO:0044822]  
2
  • IDAUniProtKB
Protein bindingdefinition[GO:0005515]  
4
  • IPIIntAct
Ribonuclease III activitydefinition[GO:0004525]  
1
  • IDAWormBase
GO biological process 
Defense response to Gram-negative bacteriumdefinition[GO:0050829]  
1
  • IDAUniProtKB
Defense response to Gram-positive bacteriumdefinition[GO:0050830]  
1
  • IDAUniProtKB
miRNA metabolic processdefinition[GO:0010586] silver  
  • IEAOrtholog Compara
Pre-miRNA processingdefinition[GO:0031054] silver  
  • IEAOrtholog Compara
Primary miRNA processingdefinition[GO:0031053]  
2
  • IDAWormBase
Ribosome biogenesisdefinition[GO:0042254]  
  • IEAUniProtKB KW
RNA phosphodiester bond hydrolysisdefinition[GO:0090501]  
1
  • IDAGOC
RNA phosphodiester bond hydrolysis, endonucleolyticdefinition[GO:0090502]  
1
  • IDAGOC
  • IEAInterPro 2 GO
  • IEAOrtholog Compara
  • IEAEC 2 GO
rRNA catabolic processdefinition[GO:0016075] silver  
  • IEAInterPro 2 GO
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 3.1.26.3: Endonucleolytic cleavage to 5'-phosphomonoester.  
  • CuratedUniProtKB
It requires the following cofactor
Magnesium or manganese (By similarity).  
  • CuratedUniProtKB
 
More information is available from:
Pathways 
According to KEGG, this protein belongs to the following pathway:
Ribosome biogenesis in eukaryotes  hsa03008+29102  
According to Reactome, this protein belongs to the following pathway:
MicroRNA (miRNA) biogenesis  REACT_12417  
 

Keywords

Biological process 
RNA-mediated gene silencing  definition   [KW-0943]
Ribosome biogenesis  definition   [KW-0690]
Molecular function 
Endonuclease  definition   [KW-0255]
Hydrolase  definition   [KW-0378]
Nuclease  definition   [KW-0540]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: RNASEN
GenomeRNAi: 29102
PRO: PR:Q9NRR4