PUF60 - Poly(U)-binding-splicing factor PUF60 - human protein (Function)
 
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PUF60 »  Poly(U)-binding-splicing factor PUF60
 
Gene name:  PUF60
Family name: RRM half pint
Entry whose protein(s) existence is based on evidence at protein level
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GENE REF ISO

Function

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Overview 
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells.  
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  • CuratedUniProtKB
Isoform  Iso 6   May contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.  
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  • CuratedUniProtKB
Isoform  Iso 6   Failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer.  
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  • CuratedUniProtKB
GO molecular function 
DNA bindingdefinition[GO:0003677]  
  • IEAUniProtKB KW
Identical protein bindingdefinition[GO:0042802]  
3
  • IPIIntAct
Nucleotide bindingdefinition[GO:0000166] silver  
  • IEAInterPro 2 GO
Poly(A) RNA bindingdefinition[GO:0044822]  
2
  • IDAUniProtKB
Protein bindingdefinition[GO:0005515]  
7
  • IPIIntAct
GO biological process 
Apoptotic processdefinition[GO:0006915]  
  • IEAUniProtKB KW
mRNA processingdefinition[GO:0006397]  
  • IEAUniProtKB KW
Regulation of transcription, DNA-templateddefinition[GO:0006355]  
  • IEAUniProtKB KW
RNA splicingdefinition[GO:0008380]  
  • IEAUniProtKB KW
Transcription, DNA-templateddefinition[GO:0006351]  
  • IEAUniProtKB KW
Pathways 
According to KEGG, this protein belongs to the following pathway:
Spliceosome  hsa03040+22827  
Note 
Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells.  
  • CuratedUniProtKB
 

Keywords

Biological process 
Apoptosis  definition   [KW-0053]
Transcription  definition   [KW-0804]
Transcription regulation  definition   [KW-0805]
mRNA processing  definition   [KW-0507]
mRNA splicing  definition   [KW-0508]
Molecular function 
Repressor  definition   [KW-0678]
Ribonucleoprotein  definition   [KW-0687]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: PUF60
GenomeRNAi: 22827
PRO: PR:Q9UHX1