GNE - Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase - human protein (Function)
 
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GNE »  Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
 
Protein also known as:  UDP-GlcNAc-2-epimerase/ManAc kinase.
Gene name:  GNE
Family names: In the N-terminal section UDP-N-acetylglucosamine 2-epimerase
In the C-terminal section ROK (nagC/xylR)
Entry whose protein(s) existence is based on evidence at protein level
extend overview
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GENE REF ISO

Function

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Overview 
Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.  
2
  • UniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
1
  • UniProtKB KW
Hydrolase activity, hydrolyzing O-glycosyl compoundsdefinition[GO:0004553] silver  
1
  • InterPro 2 GO
Metal ion bindingdefinition[GO:0046872]  
1
  • UniProtKB KW
Protein bindingdefinition[GO:0005515]  
1
  • IntAct
GO biological process 
Carbohydrate phosphorylationdefinition[GO:0046835]  
3
  • GOC
  • Ortholog Compara
  • EC 2 GO
Cell adhesiondefinition[GO:0007155]  
1
  • PINC
N-acetylglucosamine biosynthetic processdefinition[GO:0006045]  
1
  • UniPathway
N-acetylneuraminate metabolic processdefinition[GO:0006054]  
1
  • PINC
UDP-N-acetylglucosamine metabolic processdefinition[GO:0006047] silver  
1
  • InterPro 2 GO
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC 3.2.1.183: UDP-N-acetyl-alpha-D-glucosamine + H(2)O N-acetyl-D-mannosamine + UDP.  
1
  • UniProtKB
EC 2.7.1.60: ATP + N-acyl-D-mannosamine ADP + N-acyl-D-mannosamine 6-phosphate.  
1
  • UniProtKB
It is regulated in the following manner
Allosterically regulated (Probable); feedback inhibited by cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), the end product of neuraminic acid biosynthesis. Activity is dependent on oligomerization. The monomer is inactive, whereas the dimer catalyzes only the phosphorylation of N-acetylmannosamine; the hexamer is fully active for both enzyme activities (By similarity). Up-regulated after PKC-dependent phosphorylation.  
2
  • UniProtKB
 
More information is available from:
Pathways 
This protein is involved in the following pathway
Amino-sugar metabolism; N-acetylneuraminate biosynthesis  
1
  • UniProtKB
According to KEGG, this protein belongs to the following pathways:
Amino sugar and nucleotide sugar metabolism  hsa00520+10020  
Metabolic pathways  hsa01100+10020  
According to Reactome, this protein belongs to the following pathway:
Sialic acid metabolism  REACT_264366  
 

Keywords

Molecular function 
Hydrolase  definition   [KW-0378]
Kinase  definition   [KW-0418]
Transferase  definition   [KW-0808]
Technical term 
Allosteric enzyme  definition   [KW-0021]
Multifunctional enzyme  definition   [KW-0511]
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: GNE_(gene)
GenomeRNAi: 10020
PRO: PR:Q9Y223