GNE - Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase - human protein (Function)
 
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GNE »  Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
 
Protein also known as:  UDP-GlcNAc-2-epimerase/ManAc kinase.
Gene name:  GNE
Family names: In the N-terminal section UDP-N-acetylglucosamine 2-epimerase
In the C-terminal section ROK (nagC/xylR)
Entry whose protein(s) existence is based on evidence at protein level
extend overview
1 76 5
GENE REF ISO

Function

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Overview 
Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.  
1
  • CuratedUniProtKB
GO molecular function 
ATP bindingdefinition[GO:0005524]  
  • IEAUniProtKB KW
Hydrolase activitydefinition[GO:0016787]  
  • IEAUniProtKB KW
Metal ion bindingdefinition[GO:0046872]  
  • IEAUniProtKB KW
N-acylmannosamine kinase activitydefinition[GO:0009384]  
  • IEAEC 2 GO
UDP-N-acetylglucosamine 2-epimerase activitydefinition[GO:0008761] silver  
  • IEAOrtholog Compara
GO biological process 
Carbohydrate phosphorylationdefinition[GO:0046835]  
1
  • TASGOC
  • IEAInterPro 2 GO
  • IEAEC 2 GO
  • IEAOrtholog Compara
Cell adhesiondefinition[GO:0007155]  
1
  • TASPINC
Lipopolysaccharide biosynthetic processdefinition[GO:0009103] silver  
  • IEAInterPro 2 GO
N-acetylglucosamine biosynthetic processdefinition[GO:0006045]  
  • IEAUniPathway
N-acetylneuraminate biosynthetic processdefinition[GO:0046380] silver  
  • IEAInterPro 2 GO
N-acetylneuraminate metabolic processdefinition[GO:0006054]  
1
  • TASPINC
UDP-N-acetylglucosamine metabolic processdefinition[GO:0006047] silver  
  • IEAInterPro 2 GO
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reactions
EC 3.2.1.183: UDP-N-acetyl-alpha-D-glucosamine + H(2)O N-acetyl-D-mannosamine + UDP.  
  • CuratedUniProtKB
EC 2.7.1.60: ATP + N-acyl-D-mannosamine ADP + N-acyl-D-mannosamine 6-phosphate.  
  • CuratedUniProtKB
It is regulated in the following manner
Allosterically regulated (Probable); feedback inhibited by cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac), the end product of neuraminic acid biosynthesis. Activity is dependent on oligomerization. The monomer is inactive, whereas the dimer catalyzes only the phosphorylation of N-acetylmannosamine; the hexamer is fully active for both enzyme activities (By similarity). Up-regulated after PKC-dependent phosphorylation.  
1
  • CuratedUniProtKB
Pathways 
This protein is involved in the following pathway
According to KEGG, this protein belongs to the following pathways:
Amino sugar and nucleotide sugar metabolism  hsa00520+10020  
Metabolic pathways  hsa01100+10020  
According to Reactome, this protein belongs to the following pathway:
Sialic acid metabolism  REACT_200874  
 

Keywords

Molecular function 
Hydrolase  definition   [KW-0378]
Kinase  definition   [KW-0418]
Transferase  definition   [KW-0808]
Technical term 
Allosteric enzyme  definition   [KW-0021]
Multifunctional enzyme  definition   [KW-0511]
Reference proteome  definition   [KW-1185]
 

Further external links

GeneWiki: GNE_(gene)
GenomeRNAi: 10020
PRO: PR:Q9Y223