POFUT2 - GDP-fucose protein O-fucosyltransferase 2 - human protein (Function)
 
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POFUT2 »  GDP-fucose protein O-fucosyltransferase 2   [ EC 2.4.1.221 ]
 
Protein also known as:  Peptide-O-fucosyltransferase 2 (O-FucT-2).
Gene name:  POFUT2
Family name: Glycosyltransferase 68
Entry whose protein(s) existence is based on evidence at protein level
extend overview
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GENE REF ISO

Function

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Overview 
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type 1 repeats where C1 and C2 are the first and second cysteines, respectively. O-fucosylates members of several protein families including the ADAMTS family, the thrombosporin (TSP) and spondin families. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity). Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13.  
5
  • CuratedUniProtKB
GO molecular function 
Peptide-O-fucosyltransferase activitydefinition[GO:0046922]  
1
  • IDAUniProtKB
GO biological process 
Fucose metabolic processdefinition[GO:0006004]  
  • IEAUniProtKB KW
Mesoderm formationdefinition[GO:0001707] silver  
  • IEAOrtholog Compara
Protein O-linked fucosylationdefinition[GO:0036066]  
1
  • IDAUniProtKB
Regulation of epithelial to mesenchymal transitiondefinition[GO:0010717] silver  
  • IEAOrtholog Compara
Regulation of gene expressiondefinition[GO:0010468] silver  
  • IEAOrtholog Compara
Regulation of secretiondefinition[GO:0051046]  
1
  • IDAUniProtKB
Enzymatic activity 
This protein acts as an enzyme. It is known to catalyze the following reaction
EC 2.4.1.221: Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.  
1
  • CuratedUniProtKB
It is regulated in the following manner
Inhibited by EDTA and by Zn(2+).  
1
  • CuratedUniProtKB
According to CAZy, this protein is belongs to the following pathway:
Glycosyltransferase Family 68  GT68  
Pathways 
This protein is involved in the following pathway
According to KEGG, this protein belongs to the following pathway:
Other types of O-glycan biosynthesis  hsa00514+23275  
 

Biophysicochemical properties

Kinetic parameters
KM 9.8 uM for GDP-fucose

Keywords

Biological process 
Carbohydrate metabolism  definition   [KW-0119]
Fucose metabolism  definition   [KW-0294]
Molecular function 
Glycosyltransferase  definition   [KW-0328]
Transferase  definition   [KW-0808]
Technical term 
Reference proteome  definition   [KW-1185]
 

Further external links

GenomeRNAi: 23275
PRO: PR:Q9Y2G5